Brief Report

Archives of Virology

, Volume 153, Issue 10, pp 1955-1960

First online:

Cleavage sites of the “P3 region” in the nonstructural polyprotein precursor of a dicistrovirus

  • Nobuhiko NakashimaAffiliated withNational Institute of Agrobiological Sciences Email author 
  • , Yuri NakamuraAffiliated withNational Institute of Agrobiological Sciences

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Abstract

Plautia stali intestine virus (PSIV) is a member of the family Dicistroviridae. Dicistroviruses, like picornaviruses, are thought to encode a 3C-like protease; however, the cleavage sites of dicistroviral nonstructural polyprotein precursors are unknown except for those in genome-linked virus protein (VPg) regions. We expressed part of the PSIV polyprotein in Escherichia coli and detected autoprocessed viral proteins. N-terminal sequence analysis of the autoprocessed proteins showed that Q/GWLSW and Q/NGVFD in the PSIV sequence correspond to 2C/3A and 3C/3D cleavage sites in picornaviruses. Alignments of deduced amino acid sequences of the “P3 region” suggest that these cleavage sites can be predicted.