Archives of Virology

, Volume 153, Issue 8, pp 1517–1525

Lytic induction of Kaposi’s sarcoma-associated herpesvirus in primary effusion lymphoma cells with natural products identified by a cell-based fluorescence moderate-throughput screening

  • Hye-Jeong Cho
  • Fuqu Yu
  • Ren Sun
  • Dongho Lee
  • Moon Jung Song
Original Article

DOI: 10.1007/s00705-008-0144-4

Cite this article as:
Cho, HJ., Yu, F., Sun, R. et al. Arch Virol (2008) 153: 1517. doi:10.1007/s00705-008-0144-4

Abstract

Kaposi’s sarcoma-associated herpesvirus (KSHV) has been linked to Kaposi’s sarcoma primary effusion lymphoma (PEL), and multicentric Castleman’s disease. Intentional lytic induction of gammaherpesviruses in the presence of antiviral drugs is thought to be an effective treatment option for gammaherpesvirus-related tumors. In this study, we used a cell-based fluorescence bioassay system in which a KSHV-infected PEL cell line was stably transfected with a potent viral-promoter-driven reporter gene to identify effective non-toxic reagents capable of inducing latent KSHV. Among 400 plant extracts screened, three extracts increased reporter gene expression in a dose-dependent manner. Furthermore, the three extracts activated the RTA promoter and induced expression of lytic genes in the endogenous viral genomes of KSHV-infected tumor cells. Together, our results demonstrate the effectiveness of a moderate-throughput screening system to identify natural products capable of inducing KSHV reactivation, thereby facilitating the development of novel therapeutic agents for KSHV-associated malignancies.

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Hye-Jeong Cho
    • 1
  • Fuqu Yu
    • 2
  • Ren Sun
    • 2
  • Dongho Lee
    • 1
  • Moon Jung Song
    • 1
  1. 1.Division of Biotechnology, College of Life Sciences and BiotechnologyKorea UniversitySeoulRepublic of Korea
  2. 2.Department of Molecular and Medical PharmacologyUniversity of California at Los AngelesLos AngelesU.S.A

Personalised recommendations