Prevalence of antibody to hepatitis E virus among wild sika deer, Cervus nippon, in Japan
First Online: 13 April 2007 Received: 25 January 2007 Accepted: 22 February 2007 DOI:
Cite this article as: Matsuura, Y., Suzuki, M., Yoshimatsu, K. et al. Arch Virol (2007) 152: 1375. doi:10.1007/s00705-007-0965-6 Summary
We examined 976 sika deer serum samples, 159 liver tissue samples and 88 stool samples collected from 16 prefectures in Japan, and performed ELISA and RT-PCR assays to detect antibodies to HEV and HEV RNA, respectively. Although 25 (2.6%) of 976 samples were positive for anti-HEV IgG, the antibody titers were very low. The OD values ranged between 0.018 and 0.486, forming a single distribution rather than a bimodal distribution, suggesting that the antibody detected in this study was not induced by HEV infection, or that deer have low sensitivity to HEV. HEV RNA was not detected in these samples, also suggesting that deer may not play a role as an HEV reservoir.
References Balayan, MS 1997 Epidemiology of hepatitis E virus infection J Viral Hepat 4 155 165 PubMed CrossRef Buisson, Y, Grandadam, M, Nicand, E, Cheval, P, van Cuyck-Gandre, H, Innis, B, Rehel, P, Coursaget, P, Teyssou, R, Tsarev, S 2000 Identification of a novel hepatitis E virus in Nigeria J Gen Virol 81 903 909 PubMed
Emerson SU AD, Arankalle A, Meng XJ, Purdy M, Schlauder GG, Tsarev SA (2005) Hepevirus. In: Fauquet CM, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy, VIIIth Report of the ICTV. Elsevier/Academic Press, London, pp 853–857
Hijikata, M, Hayashi, S, Trinh, NT, Ha le, D, Ohara, H, Shimizu, YK, Keicho, N, Yoshikura, H 2002 Genotyping of hepatitis E virus from Vietnam Intervirology 45 101 104 PubMed CrossRef Jameel, S 1999 Molecular biology and pathogenesis of hepatitis E virus Expert Rev Mol Med 1999 1 16 PubMed CrossRef Koike, H, Ohtaishi, N 1985 Prehistoric hunting pressure estimated by the age composition of excavated sika deer ( Cervus nippon) using the annual layer of tooth cement J Archaeol Sci 12 443 456 CrossRef Li, TC, Yamakawa, Y, Suzuki, K, Tatsumi, M, Razak, MA, Uchida, T, Takeda, N, Miyamura, T 1997 Expression and self-assembly of empty virus-like particles of hepatitis E virus J Virol 71 7207 7213 PubMed Li, TC, Zhang, J, Shinzawa, H, Ishibashi, M, Sata, M, Mast, EE, Kim, K, Miyamura, T, Takeda, N 2000 Empty virus-like particle-based enzyme-linked immunosorbent assay for antibodies to hepatitis E virus J Med Virol 62 327 333 PubMed CrossRef Li, TC, Chijiwa, K, Sera, N, Ishibashi, T, Etoh, Y, Shinohara, Y, Kurata, Y, Ishida, M, Sakamoto, S, Takeda, N, Miyamura, T 2005 Hepatitis E virus transmission from wild boar meat Emerg Infect Dis 11 1958 1960 PubMed Li, TC, Saito, M, Ogura, G, Ishibashi, O, Miyamura, T, Takeda, N 2006 Serologic evidence for hepatitis E virus infection in mongoose Am J Trop Med Hyg 74 932 936 PubMed Magden, J, Takeda, N, Li, T, Auvinen, P, Ahola, T, Miyamura, T, Merits, A, Kaariainen, L 2001 Virus-specific mRNA capping enzyme encoded by hepatitis E virus J Virol 75 6249 6255 PubMed CrossRef Maila, HT, Bowyer, SM, Swanepoel, R 2004 Identification of a new strain of hepatitis E virus from an outbreak in Namibia in 1995 J Gen Virol 85 89 95 PubMed CrossRef Meng, XJ, Purcell, RH, Halbur, PG, Lehman, JR, Webb, DM, Tsareva, TS, Haynes, JS, Thacker, BJ, Emerson, SU 1997 A novel virus in swine is closely related to the human hepatitis E virus Proc Natl Acad Sci USA 94 9860 9865 PubMed CrossRef Meng, XJ, Halbur, PG, Shapiro, MS, Govindarajan, S, Bruna, JD, Mushahwar, IK, Purcell, RH, Emerson, SU 1998 Genetic and experimental evidence for cross-species infection by swine hepatitis E virus J Virol 72 9714 9721 PubMed Meng, XJ 2003 Swine hepatitis E virus: cross-species infection and risk in xenotransplantation Curr Top Microbiol Immunol 278 185 216 PubMed Purcell, RH, Emerson, SU 2001 Hepatitis E virus Knipe, DM Howley, PM eds. Fields virology Lippincott Williams & Wilkins Philadelphia 3051 3061 Schlauder, GG, Dawson, GJ, Erker, JC, Kwo, PY, Knigge, MF, Smalley, DL, Rosenblatt, JE, Desai, SM, Mushahwar, IK 1998 The sequence and phylogenetic analysis of a novel hepatitis E virus isolated from a patient with acute hepatitis reported in the United States J Gen Virol 79 447 456 PubMed Schlauder, GG, Mushahwar, IK 2001 Genetic heterogeneity of hepatitis E virus J Med Virol 65 282 292 PubMed CrossRef Takahashi, K, Iwata, K, Watanabe, N, Hatahara, T, Ohta, Y, Baba, K, Mishiro, S 2001 Full-genome nucleotide sequence of a hepatitis E virus strain that may be indigenous to Japan Virology 287 9 12 PubMed CrossRef Takahashi, K, Kang, JH, Ohnishi, S, Hino, K, Miyakawa, H, Miyakawa, Y, Maekubo, H, Mishiro, S 2003 Full-length sequences of six hepatitis E virus isolates of genotypes III and IV from patients with sporadic acute or fulminant hepatitis in Japan Intervirology 46 308 318 PubMed CrossRef Takahashi, M, Nishizawa, T, Miyajima, H, Gotanda, Y, Iita, T, Tsuda, F, Okamoto, H 2003 Swine hepatitis E virus strains in Japan form four phylogenetic clusters comparable with those of Japanese isolates of human hepatitis E virus J Gen Virol 84 851 862 PubMed CrossRef Takahashi, M, Nishizawa, T, Okamoto, H 2003 Identification of a genotype III swine hepatitis E virus that was isolated from a Japanese pig born in 1990 and that is most closely related to Japanese isolates of human hepatitis E virus J Clin Microbiol 41 1342 1343 PubMed CrossRef Tam, AW, Smith, MM, Guerra, ME, Huang, CC, Bradley, DW, Fry, KE, Reyes, GR 1991 Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome Virology 185 120 131 PubMed CrossRef Tei, S, Kitajima, N, Takahashi, K, Mishiro, S 2003 Zoonotic transmission of hepatitis E virus from deer to human beings Lancet 362 371 373 PubMed CrossRef Tei, S, Kitajima, N, Ohara, S, Inoue, Y, Miki, M, Yamatani, T, Yamabe, H, Mishiro, S, Kinoshita, Y 2004 Consumption of uncooked deer meat as a risk factor for hepatitis E virus infection: an age- and sex-matched case-control study J Med Virol 74 67 70 PubMed CrossRef Velazquez, O, Stetler, HC, Avila, C, Ornelas, G, Alvarez, C, Hadler, SC, Bradley, DW, Sepulveda, J 1990 Epidemic transmission of enterically transmitted non-A, non-B hepatitis in Mexico, 1986–1987 JAMA 263 3281 3285 PubMed CrossRef Wang, Y, Ling, R, Erker, JC, Zhang, H, Li, H, Desai, S, Mushahwar, IK, Harrison, TJ 1999 A divergent genotype of hepatitis E virus in Chinese patients with acute hepatitis J Gen Virol 80 169 177 PubMed Wang, Y, Zhang, H, Ling, R, Li, H, Harrison, TJ 2000 The complete sequence of hepatitis E virus genotype 4 reveals an alternative strategy for translation of open reading frames 2 and 3 J Gen Virol 81 1675 1686 PubMed Wu, JC, Chen, CM, Chiang, TY, Tsai, WH, Jeng, WJ, Sheen, IJ, Lin, CC, Meng, XJ 2002 Spread of hepatitis E virus among different-aged pigs: two-year survey in Taiwan J Med Virol 66 488 492 PubMed CrossRef Yazaki, Y, Mizuo, H, Takahashi, M, Nishizawa, T, Sasaki, N, Gotanda, Y, Okamoto, H 2003 Sporadic acute or fulminant hepatitis E in Hokkaido, Japan, may be food-borne, as suggested by the presence of hepatitis E virus in pig liver as food J Gen Virol 84 2351 2357 PubMed CrossRef