Archives of Virology

, Volume 152, Issue 1, pp 85–96

Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept

  • A. Hassani-Mehraban
  • J. Saaijer
  • D. Peters
  • R. Goldbach
  • R. Kormelink
Article

DOI: 10.1007/s00705-006-0827-7

Cite this article as:
Hassani-Mehraban, A., Saaijer, J., Peters, D. et al. Arch Virol (2007) 152: 85. doi:10.1007/s00705-006-0827-7

Summary.

Two strains of Tomato yellow ring virus (TYRV, genus Tospovirus), one from tomato (referred to as TYRV-t) and the other from soybean and potato (denoted TYRV-s), collected from different geographical regions in Iran, were compared. Their genomic S RNA segments differed in size by 55 nucleotides. Comparison of the S RNA intergenic regions revealed the absence of a stretch of 115 nucleotides within the S RNA segment of TYRV-s and, conversely, of 56 nts in that of TYRV-t, apparently a stable genetic difference as it was also found in another isolate of TYRV-s collected from potato. Sequence comparison of the N protein ORFs revealed an identity of 92% between the N proteins of both strains, and the observed strong cross-reaction of TYRV-s in DAS-ELISA with a polyclonal antiserum directed against the TYRV-t N protein confirmed this high identity. Host range analysis revealed several differences, e.g. TYRV-s, but not TYRV-t, being able to systemically infect Nicotiana species, and TYRV-s being localised in tomato. The observed molecular and biological differences of both viruses call into question the currently used criteria for Tospovirus species demarcation.

Copyright information

© Springer-Verlag 2006

Authors and Affiliations

  • A. Hassani-Mehraban
    • 1
  • J. Saaijer
    • 1
  • D. Peters
    • 1
  • R. Goldbach
    • 1
  • R. Kormelink
    • 1
  1. 1.Laboratory of VirologyWageningen UniversityWageningenThe Netherlands