Brief Report

Archives of Virology

, Volume 151, Issue 10, pp 2047-2053

First online:

The activation of lytic replication of Epstein-Barr virus by baculovirus-mediated gene transduction

  • L. WangAffiliated withDepartment of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University
  • , L. ShanAffiliated withDepartment of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University
  • , J. YinAffiliated withDepartment of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University
  • , M. ZhaoAffiliated withDepartment of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University
  • , D. SuAffiliated withDepartment of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University
  • , J. ZhongAffiliated withDepartment of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University

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Summary.

A baculoviral mammalian-cell vector was constructed to express Rta, a protein of Epstein-Barr virus (EBV) responsible for the transition from latent infection to lytic replication. EBV lytic replication and cell-growth inhibition was observed in infected D98/HR1 cells. The baculovirus caused little cytotoxicity in the non-targeted HeLa cells, compared to an adenovirus vector. It is concluded that recombinant baculovirus might have the potential as a vector for the therapy of EBV-related cancer.