Molecular epidemiology of rabies from Maranhão and surrounding states in the northeastern region of Brazil Authors
First Online: 16 June 2006 Received: 05 February 2006 Accepted: 27 March 2006 DOI:
Cite this article as: Sato, G., Kobayashi, Y., Shoji, Y. et al. Arch Virol (2006) 151: 2243. doi:10.1007/s00705-006-0770-7 Summary.
Although many outbreaks of rabies have been reported in northern Brazil, few epidemiological studies of these outbreaks have been undertaken. In this study, molecular epidemiological analyses were performed using 41 rabies virus samples isolated in the Maranhão (MA), Pará (PA), and Tocantins (TO) states of northeastern Brazil. A 599-bp region of the glycoprotein (G) gene was first amplified from each sample by RT-PCR, then sequenced and subjected to phylogenetic analysis. A phylogenetic tree divided the 41 isolates into two clades: Clade I was associated with terrestrial carnivores and Clade II was associated with vampire bats. The Clade I isolates were further sub-divided into two groups. The first group was closer to carnivore isolates that predominate in central Brazil, whereas the second group more closely resembled wild fox isolates from the northeastern coastal state of Paraíba (PB). MA isolates of Clade II formed an entirely separate group. These results demonstrate that bat- and dog-transmitted rabies occur in northwestern Brazil.
Agência Estadual de Defesa Agropecuária do Maranhão (2005) Governo do maranhão recebe reforço de São Paulo contra a raiva. Retrieved from
Accessed February 4, 2006
Batista da Costa, M, Bonito, RF, Nishioka, SA 1993 An outbreak of vampire bat bite in a Brazilian village Trop Med Parasitol 44 219 220 PubMed Batista-Morais, N, Neilson-Rolim, B, Matos-Chaves, HH, de Brito-Neto, J, Maria-da-Silva, L 2000 Rabies in tamarins ( Callithrix jacchus) in the state of Ceara, Brazil, a distinct viral variant? Mem Inst Oswaldo Cruz 95 609 610 PubMed CrossRef Bernardi, F, Nadin-Davis, SA, Wandeler, AI, Armstrong, J, Gomes, AA, Lima, FS, Nogueira, FR, Ito, FH 2005 Antigenic and genetic characterization of rabies viruses isolated from domestic and wild animals of Brazil identifies the hoary fox as a rabies reservoir J Gen Virol 86 3153 3162 PubMed CrossRef Cisterna, D, Bonaventura, R, Caillou, S, Pozo, O, Andreau, ML, Fontana, LD, Echegoyen, C, de Mattos, C, de Mattos, C, Russo, S, Novaro, L, Elberger, D, Freire, MC 2005 Antigenic and molecular characterization of rabies virus in Argentina Virus Res 109 139 147 PubMed CrossRef De Mattos, CC, De Mattos, CA, Loza-Rubio, E, Aguilar-Setien, A, Orciari, LA, Smith, JS 1999 Molecular characterization of rabies virus isolates from Mexico: implications for transmission dynamics and human risk Am J Trop Med Hyg 61 587 597 PubMed Goncalves, MA, Sa-Neto, RJ, Brazil, TK 2002 Outbreak of aggressions and transmission of rabies in human beings by vampire bats in northeastern Brazil Rev Soc Bras Med Trop 35 461 464 PubMed Heinemann, MB, Fernandes-Matioli, FM, Cortez, A, Soares, RM, Sakamoto, SM, Bernardi, F, Ito, FH, Madeira, AM, Richtzenhain, LJ 2002 Genealogical analyses of rabies virus strains from Brazil based on N gene alleles Epidemiol Infect 128 503 511 PubMed CrossRef Ito, M, Arai, YT, Itou, T, Sakai, T, Ito, FH, Takasaki, T, Kurane, I 2001 Genetic char- acterization and geographic distribution of rabies virus isolates in Brazil: identification of two reservoirs, dogs and vampire bats Virology 284 214 222 PubMed CrossRef Mayern, F 2003 Haematophagous bats in Brazil, their role in rabies transmission, impact on public health, livestock industry and alternatives to an indiscriminate reduction of bat population J Vet Med B 50 469 472 CrossRef Paezs, A, Saad, C, Nunez, C, Boshell, J 2005 Molecular epidemiology of rabies in northern Colombia 1994–2003. Evidence for human and fox rabies associated with dogs Epidemiol Infect 133 529 536 CrossRef Sato, G, Itou, T, Shoji, Y, Miura, Y, Mikami, T, Ito, M, Kurane, I, Samara, SI, Carvalho, AA, Nociti, DP, Ito, FH, Sakai, T 2004 Genetic and phylogenetic analysis of glycoprotein of rabies virus isolated from several species in Brazil J Vet Med Sci 66 747 753 PubMed CrossRef Schneider, MC, Aron, J, Santos-Burgoa, C, Uieda, W, Ruiz-Velazco, S 2001 Common vampire bat attacks on humans in a village of the Amazon region of Brazil Cad Saude Publica 17 1531 1536 PubMed CrossRef Schneider, MC, Santos-Burgoa, C, Aron, J, Munoz, B, Ruiz-Velazco, S, Uieda, W 1996 Potential force of infection of human rabies transmitted by vampire bats in the Amazonian region of Brazil Am J Trop Med Hyg 55 680 684 PubMed Schaefer, R, Batista, HB, Franco, AC, Rijsewijk, FA, Roehe, PM 2005 Studies on antigenic and genomic properties of Brazilian rabies virus isolates Vet Microbiol 107 161 170 PubMed CrossRef