Comparative sequence analysis of envelope protein genes of Indian buffalopox virus isolates Article First Online: 20 April 2006 Received: 16 January 2006 Accepted: 04 March 2006 DOI:
Cite this article as: Singh, R., Hosamani, M., Balamurugan, V. et al. Arch Virol (2006) 151: 1995. doi:10.1007/s00705-006-0761-8 Summary.
Buffalopox virus (BPXV) is considered to be a close variant of vaccinia virus (VACV), the prototype member of the genus
Orthopoxvirus. In the present study, we have analyzed the sequences of H3L, A27L, and D8L gene-homologues of VACV in BPXV to elucidate its genetic relationship to VACV and other orthopoxviruses (OPVs). Products of these genes have been shown to be important in attachment of VACV to host cell surface receptors during viral entry. Additionally, the A27L gene is also responsible for cell fusion during infection, while the H3L gene is required for synthesis of the highly immunogenic major envelope protein p35. Full-length nucleotide sequences of H3L, A27L, and D8L genes of three BPXV isolates were determined by PCR amplification, cloning, and sequencing. The nucleotide (nt) sequence and the deduced amino acid (aa) sequences were compared with published sequences from other members of the genus Orthopoxvirus. Comparative sequence analysis of all the three genes revealed high sequence identity of BPXV isolates with VACV (close to 99% sequence identity) at both the nt and aa level. Phylogenetic analysis based on the deduced aa sequences of the H3L, A27L, and D8L genes also showed that BPXVs are more closely related to VACV than to any of the other OPVs. References Altschul, SF, Madden, TL, Schäffer, AA, Zhang, J, Zhang, Z, Miller, W, Lipman, DJ 1997 Nucleic Acids Res 25 3389 3402 PubMed CrossRef Google Scholar Chandra, R, Singh, IP, Garg, SK, Varshney, KC 1986 Experimental pathogenesis of buffalo pox virus in rabbits: clinico-pathological studies Acta Virol 30 390 396 PubMed Google Scholar Chung, CS, Hsaio, JC, Chang, YS, Chang, W 1998 A27L protein mediates vaccinia virus interaction with cell surface heparan sulfate J Virol 72 1577 1585 PubMed Google Scholar Damaso, CRA, Espostio, JJ, Condit, RC, Moussatche, N 2000 An emergent poxvirus from humans and cattle in Rio de Janeiro state: Cantagalo virus may derive from Brazilian small pox vaccine Virology 277 439 449 PubMed CrossRef Google Scholar Dumbell, K, Richardson, M 1993 Virological investigations of specimens from buffaloes affected by buffalopox in Maharashtra State, India between 1985 and 1987 Arch Virol 128 257 267 PubMed CrossRef Google Scholar Esposito, J, Condit, R, Obijeski, J 1981 The preparation of orthopoxvirus DNA J Virol Methods 2 175 279 PubMed CrossRef Google Scholar
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