Archives of Virology

, Volume 151, Issue 2, pp 399–404

Expression of sapovirus virus-like particles in mammalian cells

  • T. Oka
  • G. S. Hansman
  • K. Katayama
  • S. Ogawa
  • N. Nagata
  • T. Miyamura
  • N. Takeda
Brief Report

DOI: 10.1007/s00705-005-0613-y

Cite this article as:
Oka, T., Hansman, G., Katayama, K. et al. Arch Virol (2006) 151: 399. doi:10.1007/s00705-005-0613-y

Summary.

Sapovirus (SaV) is an etiological agent of acute gastroenteritis in human and swine. SaV can be divided into five genogroups, GI to GV. Virus-like particles (VLPs) morphologically similar to native SaV have been expressed for GI, GII, GIII and GV strains in insect cells, although only low expression levels were observed for GII strains. In this study, we report the successful expression of SaV GII VLPs using cultured mammalian COS-7 and 293T cells. Our results demonstrated that this mammalian expression system was able to express and form SaV VLPs.

Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  • T. Oka
    • 1
  • G. S. Hansman
    • 1
  • K. Katayama
    • 1
  • S. Ogawa
    • 1
  • N. Nagata
    • 2
  • T. Miyamura
    • 1
  • N. Takeda
    • 1
  1. 1.Department of Virology IINational Institute of Infectious DiseasesTokyoJapan
  2. 2.Department of PathologyNational Institute of Infectious DiseasesTokyoJapan