Expression of sapovirus virus-like particles in mammalian cells Brief Report First Online: 23 August 2005 Received: 26 May 2005 Accepted: 01 July 2005 DOI:
10.1007/s00705-005-0613-y Cite this article as: Oka, T., Hansman, G., Katayama, K. et al. Arch Virol (2006) 151: 399. doi:10.1007/s00705-005-0613-y Summary.
Sapovirus (SaV) is an etiological agent of acute gastroenteritis in human and swine. SaV can be divided into five genogroups, GI to GV. Virus-like particles (VLPs) morphologically similar to native SaV have been expressed for GI, GII, GIII and GV strains in insect cells, although only low expression levels were observed for GII strains. In this study, we report the successful expression of SaV GII VLPs using cultured mammalian COS-7 and 293T cells. Our results demonstrated that this mammalian expression system was able to express and form SaV VLPs.
References Chang, KO, Sosnovtsev, SV, Belliot, G, Kim, Y, Saif, LJ, Green, KY 2004 Bile acids are essential for porcine enteric calicivirus replication in association with down-regulation of signal transducer and activator of transcription 1. Proc Natl Acad Sci USA 101 8733 8738 PubMed Google Scholar Chen, R, Neill, JD, Noel, JS, Hutson, AM, Glass, RI, Estes, MK, Prasad, BV 2004 Inter- and intragenus structural variations in caliciviruses and their functional implications. J Virol 78 6469 6479 PubMed Google Scholar Clarke, IN, Lambden, PR 2000 Organization and expression of calicivirus genes. J Infect Dis 181 309 316 Google Scholar Farkas, T, Zhong, WM, Jing, Y, Huang, PW, Espinosa, SM, Martinez, N, Morrow, AL, Ruiz-Palacios, GM, Pickering, LK, Jiang, X 2004 Genetic diversity among sapoviruses. Arch Virol 149 1309 1323 CrossRef PubMed Google Scholar Flynn, WT, Saif, LJ 1988 Serial propagation of porcine enteric calicivirus-like virus in primary porcine kidney cell cultures. J Clin Microbiol 26 206 212 PubMed Google Scholar Fuerst, TR, Niles, EG, Studier, FW, Moss, B 1986 Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci USA 83 8122 8126 PubMed Google Scholar Green, KY, Ando, T, Balayan, MS, Berke, T, Clarke, IN, Estes, MK, Matson, DO, Nakata, S, Neill, JD, Studdert, MJ, Thiel, HJ 2000 Taxonomy of the caliciviruses. J Infect Dis 181 S322 S330 PubMed Google Scholar Guo, M, Qian, Y, Chang, KO, Saif, LJ 2001 Expression and self-assembly in baculovirus of porcine enteric calicivirus capsids into virus-like particles and their use in an enzyme-linked immunosorbent assay for antibody detection in swine. J Clin Microbiol 39 1487 1493 CrossRef PubMed Google Scholar Hansman, GS, Katayama, K, Maneekarn, N, Peerakome, S, Khamrin, P, Tonusin, S, Okitsu, S, Nishio, O, Takeda, N, Ushijima, H 2004 Genetic diversity of norovirus and sapovirus in hospitalized infants with sporadic cases of acute gastroenteritis in Chiang Mai, Thailand. J Clin Microbiol 42 1305 1307 CrossRef PubMed Google Scholar Hansman, GS, Katayama, K, Oka, T, Natori, K, Takeda, N 2005 Mutational study of sapovirus expression in insect cells. Virol J 2 13 CrossRef PubMed Google Scholar Hansman, GS, Natori, K, Oka, T, Ogawa, S, Tanaka, K, Nagata, N, Ushijima, H, Takeda, N, Katayama, K 2005 Cross-reactivity among sapovirus recombinant capsid proteins. Arch Virol 150 21 36 PubMed Google Scholar Hansman, GS, Natori, K, Ushijima, H, Katayama, K, Takeda, N 2005 Characterization of polyclonal antibodies raised against sapovirus genogroup five virus-like particles. Arch Virol 150 1433 1437 PubMed Google Scholar Jiang, X, Zhong, W, Kaplan, M, Pickering, LK, Matson, DO 1999 Expression and characterization of Sapporo-like human calicivirus capsid proteins in baculovirus. J Virol Methods 78 81 91 CrossRef PubMed Google Scholar Katayama, K, Miyoshi, T, Uchino, K, Oka, T, Tanaka, T, Takeda, N, Hansman, GS 2004 Novel recombinant sapovirus. Emerg Infect Dis 10 1874 1876 PubMed Google Scholar Mayo, MA 2002 A summary of taxonomic changes recently approved by ICTV. Arch Virol 147 1655 1663 PubMed Google Scholar Numata, K, Hardy, ME, Nakata, S, Chiba, S, Estes, MK 1997 Molecular characterization of morphologically typical human calicivirus Sapporo. Arch Virol 142 1537 1552 CrossRef PubMed Google Scholar Oka, T, Katayama, K, Ogawa, S, Hansman, GS, Kageyama, T, Ushijima, H, Miyamura, T, Takeda, N 2005 Proteolytic processing of sapovirus ORF1 polyprotein. J Virol 79 7283 7290 CrossRef PubMed Google Scholar Robinson, S, Clarke, IN, Vipond, IB, Caul, EO, Lambden, PR 2002 Epidemiology of human Sapporo-like caliciviruses in the South West of England: molecular characterisation of a genetically distinct isolate. J Med Virol 67 282 288 CrossRef PubMed Google Scholar Schuffenecker, I, Ando, T, Thouvenot, D, Lina, B, Aymard, M 2001 Genetic classification of “Sapporo-like viruses”. Arch Virol 146 2115 2132 CrossRef PubMed Google Scholar Copyright information
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