Archives of Virology

, Volume 150, Issue 7, pp 1485–1491

Characterization of a vaccinia virus strain used to produce smallpox vaccine in Argentina between 1937 and 1970

Authors

  • A. Lewis
    • Virology DepartmentNational Institute of Infectious Diseases, Buenos Aires, Argentina, ANLIS
  • K. Bok
    • Virology DepartmentNational Institute of Infectious Diseases, Buenos Aires, Argentina, ANLIS
  • O. Perez
    • National Institute of Biological Production, ANLIS
  • J. DeFillippo
    • National Institute of Biological Production, ANLIS
  • C. Paolazzi
    • National Institute of Biological Production, ANLIS
  • J. A. Gomez
    • Virology DepartmentNational Institute of Infectious Diseases, Buenos Aires, Argentina, ANLIS
Brief Report

DOI: 10.1007/s00705-005-0498-9

Cite this article as:
Lewis, A., Bok, K., Perez, O. et al. Arch Virol (2005) 150: 1485. doi:10.1007/s00705-005-0498-9

Summary.

Due to recent political developments, smallpox has re-emerged as a serious threat. We recovered and characterized an old batch of smallpox vaccine, Malbrán strain, produced between 1945 and 1949. The virus was re-isolated and characterized by sequence analysis and biological activity in animals. Phylogenetic analysis using the hemagglutinin and A45R genes showed that the Malbrán strain was closely related to the Lister strain of vaccinia virus. In animals, the Malbrán strain exhibited low pathogenicity, confirming historical records. Mice immunized with the Malbrán strain survived a lethal challenge with cowpox virus. Thus, this strain of vaccinia virus remains a viable candidate as a smallpox vaccine.

Copyright information

© Springer-Verlag/Wien 2005