Archives of Virology

, Volume 149, Issue 9, pp 1851–1857

Competition between wild-type virus and a reassortant from subgroups I and II of CMV and activation of antiviral responses in cowpea

Authors

  • M. Takeshita
    • Laboratory of Plant Pathology, Faculty of AgricultureGraduate School of Kyushu University
  • K. Kikuhara
    • Fukuoka Agricultural Research Center
  • S. Kuwata
    • Laboratory of Plant Cell Technology, Department of Life SciencesSchool of Agriculture, Meiji University
  • N. Furuya
    • Laboratory of Plant Pathology, Faculty of AgricultureGraduate School of Kyushu University
  • Y. Takanami
    • Laboratory of Plant Pathology, Faculty of AgricultureGraduate School of Kyushu University
Brief Report

DOI: 10.1007/s00705-004-0332-9

Cite this article as:
Takeshita, M., Kikuhara, K., Kuwata, S. et al. Arch Virol (2004) 149: 1851. doi:10.1007/s00705-004-0332-9

Summary.

To investigate the interactions between RNA3 and RNA4 from subgroups I and II in mixed infections, accumulation of CMV RNA were analyzed. In the mixed inoculation assays with CMV-LE (LE, subgroup I) and a reassortant LLm consisting of RNA1 and RNA2 from LE, and RNA3 from CMV-m2 (m2, subgroup II), LE RNA3 and RNA4 could systemically spread in the plants, whereas those of m2 could not. Furthermore, accumulation of virus short RNA and a cowpea-encoded RNA-directed RNA polymerase gene (VuRdRP1) mRNA were found in the plants, suggesting that VIGS and/or distinct antiviral responses (was) were activated by infection with CMV.

Copyright information

© Springer-Verlag/Wien 2004