Archives of Virology

, Volume 149, Issue 3, pp 633–640

Complete nucleotide sequence of Oat necrotic mottle virus: A distinct Tritimovirus species (family Potyviridae) most closely related to Wheat streak mosaic virus

Authors

  • D. C. Stenger
    • United States Department of Agriculture, Agricultural Research Service and Department of Plant PathologyUniversity of Nebraska
  • R. French
    • United States Department of Agriculture, Agricultural Research Service and Department of Plant PathologyUniversity of Nebraska
Brief Report

DOI: 10.1007/s00705-003-0237-z

Cite this article as:
Stenger, D. & French, R. Arch Virol (2004) 149: 633. doi:10.1007/s00705-003-0237-z

Summary.

The RNA genome (9346 nucleotides) of Oat necrotic mottle virus (ONMV) was cloned and sequenced. Complete genome comparisons indicated that ONMV, currently classified as a rymovirus, was most closely related (∼73% nucleotide, ∼79% amino acid identity) to the tritimovirus Wheat streak mosaic virus (WSMV). ONMV encoded a single polyprotein, with proteinase cleavage sites very similar to those of WSMV. Pairwise comparison of ONMV and WSMV cistrons revealed that P3 was most conserved (∼79% nucleotide, ∼86% amino acid), whereas HC-Pro was most divergent (∼67% nucleotide, ∼69% amino acid). In contrast, the ONMV sequence was distantly related (∼40% nt, ∼26% amino acid identity) to that of the rymovirusRyegrass mosaic virus, with highest sequence conservation noted within the NIb cistron (∼47% nucleotide, ∼41% amino acid identity). These results firmly establish that ONMV is not a rymovirus but is instead a distinct species of the genus Tritimovirus.

Copyright information

© Springer-Verlag/Wien 2003