Brief Report

Archives of Virology

, Volume 149, Issue 3, pp 633-640

First online:

Complete nucleotide sequence of Oat necrotic mottle virus: A distinct Tritimovirus species (family Potyviridae) most closely related to Wheat streak mosaic virus

  • D. C. StengerAffiliated withUnited States Department of Agriculture, Agricultural Research Service and Department of Plant Pathology, University of Nebraska
  • , R. FrenchAffiliated withUnited States Department of Agriculture, Agricultural Research Service and Department of Plant Pathology, University of Nebraska

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Summary.

The RNA genome (9346 nucleotides) of Oat necrotic mottle virus (ONMV) was cloned and sequenced. Complete genome comparisons indicated that ONMV, currently classified as a rymovirus, was most closely related (∼73% nucleotide, ∼79% amino acid identity) to the tritimovirus Wheat streak mosaic virus (WSMV). ONMV encoded a single polyprotein, with proteinase cleavage sites very similar to those of WSMV. Pairwise comparison of ONMV and WSMV cistrons revealed that P3 was most conserved (∼79% nucleotide, ∼86% amino acid), whereas HC-Pro was most divergent (∼67% nucleotide, ∼69% amino acid). In contrast, the ONMV sequence was distantly related (∼40% nt, ∼26% amino acid identity) to that of the rymovirus Ryegrass mosaic virus, with highest sequence conservation noted within the NIb cistron (∼47% nucleotide, ∼41% amino acid identity). These results firmly establish that ONMV is not a rymovirus but is instead a distinct species of the genus Tritimovirus.