Journal of Neural Transmission

, Volume 121, Issue 2, pp 113–117

Mild hypothermia protects against oxygen glucose deprivation (OGD)-induced cell death in brain slices from adult mice

  • Mar Hernández-Guillamon
  • Laura Ortega
  • Cristina Merino-Zamorano
  • Mireia Campos-Martorell
  • Anna Rosell
  • Joan Montaner
Translational Neurosciences - Short communication

DOI: 10.1007/s00702-013-1090-1

Cite this article as:
Hernández-Guillamon, M., Ortega, L., Merino-Zamorano, C. et al. J Neural Transm (2014) 121: 113. doi:10.1007/s00702-013-1090-1

Abstract

Most of neuroprotective strategies in stroke have failed to move from bench to bedside. One explanation might be the use of excessive uniform and smooth experimental models. Therefore, we have employed a more stringent in vitro model based on cultured brain slices from adult mice submitted to OGD. Using this acute model, we have confirmed that mild hypothermia protects against OGD-induced cell death when cooling the tissue during and after OGD, but not when hypothermia is induced only during reoxygenation.

Keywords

Brain slicesOxygen glucose deprivationOGDStrokeCerebral ischemiaHypothermia

Abbreviations

OGD

Oxygen glucose deprivation

PBS

Phosphate buffered saline

LDH

Lactate dehydrogenase

MTT

3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

PI

Propidium iodide

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Mar Hernández-Guillamon
    • 1
  • Laura Ortega
    • 1
  • Cristina Merino-Zamorano
    • 1
  • Mireia Campos-Martorell
    • 1
  • Anna Rosell
    • 1
  • Joan Montaner
    • 1
    • 2
  1. 1.Neurovascular Research Laboratory, Institut de Recerca, Vall d’Hebron HospitalUniversitat Autònoma de BarcelonaBarcelonaSpain
  2. 2.Neurovascular Unit, Neurology Department, Vall d’Hebron HospitalUniversitat Autònoma de BarcelonaBarcelonaSpain