Journal of Neural Transmission

, Volume 119, Issue 4, pp 405–414

Type A monoamine oxidase is associated with induction of neuroprotective Bcl-2 by rasagiline, an inhibitor of type B monoamine oxidase

  • Keiko Inaba-Hasegawa
  • Yukihiro Akao
  • Wakako Maruyama
  • Makoto Naoi
Basic Neurosciences, Genetics and Immunology - Original Article

DOI: 10.1007/s00702-011-0730-6

Cite this article as:
Inaba-Hasegawa, K., Akao, Y., Maruyama, W. et al. J Neural Transm (2012) 119: 405. doi:10.1007/s00702-011-0730-6

Abstract

Rasagiline and (−)deprenyl (selegiline), irreversible type B monoamine oxidase (MAO-B) inhibitors, protect neuronal cells through gene induction of pro-survival Bcl-2 and neurotrophic factors in the cellular models of neurodegenerative disorders. In this paper, the role of MAO in the up-regulation of neuroprotective Bcl-2 gene by these inhibitors was studied using type A MAO (MAO-A) expressing wild SH-SY5Y cells and the transfection-enforced MAO-B overexpressed cells. Rasagiline and (−)deprenyl, and also befloxatone, a reversible MAO-A inhibitor, increased Bcl-2 mRNA and protein in SH-SY5Y cells. Silencing MAO-A expression with short interfering (si) RNA suppressed Bcl-2 induction by rasagiline, but not by (−)deprenyl. MAO-B overexpression inhibited Bcl-2 induction by rasagiline and befloxatone, but did not affect that by (−)deprenyl, suggesting the different mechanisms behind Bcl-2 gene induction by these MAO-B inhibitors. The novel role of MAO-A in Bcl-2 induction by rasagiline is discussed with regard to the molecular mechanism underlying neuroprotection by the MAO inhibitors.

Keywords

Rasagiline(−)DeprenylType A and B monoamine oxidaseNeuroprotectionBcl-2Gene induction

Abbreviations

MAO-A and MAO-B

Type A and B monoamine oxidase

MAO-B SH cells

SH-SY5Y cells overexpressed MAO-B by transfection

PD

Parkinson’s disease

siRNA

Small interfering RNA

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Keiko Inaba-Hasegawa
    • 1
  • Yukihiro Akao
    • 2
  • Wakako Maruyama
    • 3
  • Makoto Naoi
    • 1
  1. 1.Department of NeurosciencesGifu International Institute of BiotechnologyKakamigaharaJapan
  2. 2.United Graduate School of Drug Discovery and Medical Information SciencesGifu UniversityGifuJapan
  3. 3.Department of Cognitive Brain ScienceNational Research Center for Geriatrics and GerontologyObuJapan