Journal of Neural Transmission

, Volume 119, Issue 4, pp 405–414

Type A monoamine oxidase is associated with induction of neuroprotective Bcl-2 by rasagiline, an inhibitor of type B monoamine oxidase

Authors

  • Keiko Inaba-Hasegawa
    • Department of NeurosciencesGifu International Institute of Biotechnology
  • Yukihiro Akao
    • United Graduate School of Drug Discovery and Medical Information SciencesGifu University
  • Wakako Maruyama
    • Department of Cognitive Brain ScienceNational Research Center for Geriatrics and Gerontology
    • Department of NeurosciencesGifu International Institute of Biotechnology
Basic Neurosciences, Genetics and Immunology - Original Article

DOI: 10.1007/s00702-011-0730-6

Cite this article as:
Inaba-Hasegawa, K., Akao, Y., Maruyama, W. et al. J Neural Transm (2012) 119: 405. doi:10.1007/s00702-011-0730-6

Abstract

Rasagiline and (−)deprenyl (selegiline), irreversible type B monoamine oxidase (MAO-B) inhibitors, protect neuronal cells through gene induction of pro-survival Bcl-2 and neurotrophic factors in the cellular models of neurodegenerative disorders. In this paper, the role of MAO in the up-regulation of neuroprotective Bcl-2 gene by these inhibitors was studied using type A MAO (MAO-A) expressing wild SH-SY5Y cells and the transfection-enforced MAO-B overexpressed cells. Rasagiline and (−)deprenyl, and also befloxatone, a reversible MAO-A inhibitor, increased Bcl-2 mRNA and protein in SH-SY5Y cells. Silencing MAO-A expression with short interfering (si) RNA suppressed Bcl-2 induction by rasagiline, but not by (−)deprenyl. MAO-B overexpression inhibited Bcl-2 induction by rasagiline and befloxatone, but did not affect that by (−)deprenyl, suggesting the different mechanisms behind Bcl-2 gene induction by these MAO-B inhibitors. The novel role of MAO-A in Bcl-2 induction by rasagiline is discussed with regard to the molecular mechanism underlying neuroprotection by the MAO inhibitors.

Keywords

Rasagiline(−)DeprenylType A and B monoamine oxidaseNeuroprotectionBcl-2Gene induction

Abbreviations

MAO-A and MAO-B

Type A and B monoamine oxidase

MAO-B SH cells

SH-SY5Y cells overexpressed MAO-B by transfection

PD

Parkinson’s disease

siRNA

Small interfering RNA

Copyright information

© Springer-Verlag 2011