Journal of Neural Transmission

, Volume 117, Issue 6, pp 737–746

No increased chromosomal damage in l-DOPA-treated patients with Parkinson’s disease: a pilot study

  • Rajaraman Gnana Oli
  • Gholamreza Fazeli
  • Wilfried Kuhn
  • Susanne Walitza
  • Manfred Gerlach
  • Helga Stopper
Movement Disorders - Original Article

DOI: 10.1007/s00702-010-0401-z

Cite this article as:
Gnana Oli, R., Fazeli, G., Kuhn, W. et al. J Neural Transm (2010) 117: 737. doi:10.1007/s00702-010-0401-z

Abstract

Parkinson’s disease (PD) is a neurodegenerative movement disorder affecting about 2% of the human population in old age. l-3,4-Dihydroxyphenylalanine (l-DOPA) in combination with a peripheral aromatic amino acid decarboxylase inhibition has been the most frequently prescribed drug for alleviating symptoms of PD, but a potential contribution of l-DOPA therapy to further neurodegeneration via oxidative stress is still debated. We report that the specific oxidative stress biomarker 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) level in peripheral blood lymphocyte DNA was elevated to 8.1 ± 1.7 8-oxodG/106dG in 17 chronically l-DOPA-treated PD patients, compared to 4.6 ± 1.2 8-oxodG/106dG in 12 controls. However, the total antioxidative capacity of plasma was increased to 1113 ± 237 μM in the PD patients compared to 941 ± 254 μM in controls. The frequency of micronuclei, a subgroup of chromosomal aberrations, in peripheral blood lymphocytes was not elevated compared to healthy age-matched individuals. In vitro, in a cell-free assay, dopamine and its precursor l-DOPA exhibited antioxidative capacity. On the other hand, the 8-oxodG concentration in cultured PC 12 cells was enhanced after dopamine treatment. This elevation may be below a threshold for manifestation as chromosomal damage.

Keywords

Parkinson’s disease8-OxodGMicronuclei l-DOPADopamine

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Rajaraman Gnana Oli
    • 1
  • Gholamreza Fazeli
    • 1
  • Wilfried Kuhn
    • 2
  • Susanne Walitza
    • 4
  • Manfred Gerlach
    • 3
  • Helga Stopper
    • 1
  1. 1.Department of ToxicologyUniversity of WuerzburgWuerzburgGermany
  2. 2.Department of NeurologyLeopoldina-Hospital SchweinfurtSchweinfurtGermany
  3. 3.Department of Child and Adolescent Psychiatry and Psychotherapy, Psychosomatics and PsychotherapyUniversity of WuerzburgWuerzburgGermany
  4. 4.Department of Child and Adolescent PsychiatryUniversity of ZurichZurichSwitzerland