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Soil seed bank contributes significantly to genetic variation of Hypericum sinaicum in a changing environment

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Abstract

The contribution of soil seed bank of a desert endemic plant species in maintaining genetic diversity has been addressed in this paper through investigating the differences in genetic diversity and structure (using AFLP markers) between plants grown from soil seed bank and standing crop plants within and among five populations of H. sinaicum growing at St. Katherine Protectorate, southern Sinai, Egypt. Standard genetic diversity measures showed that the molecular variation within and among populations was highly significantly different between standing crop and soil seed bank. While soil seed bank had lower genetic diversity than standing crop populations, pooling soil seed bank with standing crop samples resulted in higher diversity. The results revealed also that soil seed bank had lower differentiation (7 %) than among populations of the standing crop (18 %). Results of neighbor-joining, Bayesian clustering and principal coordinate analysis showed that soil seed banks had a separate gene pool different from standing crop. The study came to the conclusion that the genetic variation of the soil seed bank contributes significantly to the genetic variation of the species. This also stresses the importance of elucidating the genetic diversity and structure of the soil seed bank for any sound and long-term conservation efforts for desert species. These have been growing in small-size populations for a long time that any estimates gained only from aboveground sampling of populations may be ambiguous.

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Acknowledgments

The authors wish to thank Alexander von Humboldt Foundation for providing funding of this project to the first author under the Georg Forster Fellowship for Experienced Researchers.

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Authors and Affiliations

  1. Botany Department, Suez Canal University, Ismailia, 41522, Egypt

    Mohamed Zaghloul

  2. Institut für Botanik, Universität Regensburg, 93053, Regensburg, Germany

    Christoph Reisch & Peter Poschlod

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  1. Mohamed Zaghloul
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  2. Christoph Reisch
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  3. Peter Poschlod
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Correspondence to Mohamed Zaghloul.

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Zaghloul, M., Reisch, C. & Poschlod, P. Soil seed bank contributes significantly to genetic variation of Hypericum sinaicum in a changing environment. Plant Syst Evol 299, 1819–1828 (2013). https://doi.org/10.1007/s00606-013-0837-3

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  • DOI: https://doi.org/10.1007/s00606-013-0837-3

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