Plant Systematics and Evolution

, Volume 284, Issue 1, pp 111–122

Intrapopulational genetic diversity of Araucaria angustifolia (Bertol.) Kuntze is different when assessed on the basis of chloroplast or nuclear markers

Original Article

DOI: 10.1007/s00606-009-0238-9

Cite this article as:
Patreze, C.M. & Tsai, S.M. Plant Syst Evol (2010) 284: 111. doi:10.1007/s00606-009-0238-9


A natural population of Araucaria angustifolia (Bertol.) Kuntze was evaluated to quantify genetic diversity, using nucleotide sequences of chloroplast maturase K genes (matK) and six specific nuclear polymorphic microsatellite loci (nuSSR). Estimates of nucleotide diversity assessed from matK sequences were low (π = 0.001), but they indicated the potential of this gene for evaluating divergence among widespread populations. The matK sequence (1,565 bp) was used for analysis of phylogenetic relationships, enabling clustering of the South American species (A. angustifolia and A. araucana). For analysis of intraspecific genetic diversity, nuSSR were more informative than matK, resulting in a polymorphic loci percentage of 69, a number average of alleles per locus of 11.6, expected genetic diversity 0.741, and significant spatial genetic structure up to 25 m. Our data can help in the selection of genetic sources for conservation of this species, which is at risk of extinction.


Araucaria angustifolia (Bertol.) Kuntze Genetic diversity Spatial structure Microsatellite cpDNA Nucleotide variation mat

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  1. 1.Centro de Energia Nuclear na AgriculturaPiracicabaBrazil

Personalised recommendations