Plant Systematics and Evolution

, Volume 282, Issue 3, pp 201–228

Phylogeny of the eudicot order Malpighiales: analysis of a recalcitrant clade with sequences of the petD group II intron

  • Nadja Korotkova
  • Julio V. Schneider
  • Dietmar Quandt
  • Andreas Worberg
  • Georg Zizka
  • Thomas Borsch
Original Article

DOI: 10.1007/s00606-008-0099-7

Cite this article as:
Korotkova, N., Schneider, J.V., Quandt, D. et al. Plant Syst Evol (2009) 282: 201. doi:10.1007/s00606-008-0099-7
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Abstract

Malpighiales are one of the most diverse orders of angiosperms. Molecular phylogenetic studies based on combined sequences of coding genes allowed to identify major lineages but hitherto were unable to resolve relationships among most families. Spacers and introns of the chloroplast genome have recently been shown to provide strong signal for inferring relationships among major angiosperm lineages and within difficult clades. In this study, we employed sequence data of the petD group II intron and the petB-petD spacer for a set of 64 Malpighiales taxa, representing all major lineages. Celastrales and Oxalidales served as outgroups. Sequence alignment was straightforward due to frequent microstructural changes with easily recognizable motifs (e.g., simple sequence repeats), and well defined mutational hotspots. The secondary structure of the complete petD intron was calculated for Idesia polycarpa as an example. Domains I and IV are the most length variable parts of the intron. They contain terminal A/T-rich stem-loop elements that are suggested to elongate independently in different lineages with a slippage mechanism earlier reported from the P8 stem-loop of the trnL intron. Parsimony and Bayesian analyses of the petD dataset yielded trees largely congruent with results from earlier multigene studies but statistical support of nodes was generally higher. For the first time a deep node of the Malpighiales backbone, a clade comprising Achariaceae, Violaceae, Malesherbiaceae, Turneraceae, Passifloraceae, and a Lacistemataceae–Salicaceae lineage received significant statistical support (83% JK, 1.00 PP) from plastid DNA sequences.

Keywords

MalpighialesAngiospermsMolecular evolutionGroup II intronsNon-coding DNA

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Nadja Korotkova
    • 1
  • Julio V. Schneider
    • 2
  • Dietmar Quandt
    • 3
    • 5
  • Andreas Worberg
    • 1
  • Georg Zizka
    • 4
  • Thomas Borsch
    • 1
    • 6
  1. 1.Nees Institute for Biodiversity of PlantsUniversity of BonnBonnGermany
  2. 2.Department of Systematic Botany, Biology IUniversity of LeipzigLeipzigGermany
  3. 3.Institute of Botany, Plant Phylogenetics and Phylogenomics GroupDresden University of TechnologyDresdenGermany
  4. 4.Department of Botany and Molecular EvolutionResearch Institute Senckenberg and Johann Wolfgang Goethe-UniversityFrankfurt am MainGermany
  5. 5.Nees Institute for Biodiversity of PlantsUniversity of BonnBonnGermany
  6. 6.Botanischer Garten und Botanisches Museum Berlin-Dahlem und Institut für BiologieFreie Universität BerlinBerlinGermany