Original Article

Plant Systematics and Evolution

, Volume 277, Issue 1, pp 13-20

Mating system and genetic diversity in natural populations of Nelumbo nucifera (Nelumbonaceae) detected by ISSR markers

  • Yan-Chuang HanAffiliated withKey Lab of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University
  • , Cai-Zhu TengAffiliated withCollege of Life Science, Guangxi Normal University
  • , Gituru Robert WahitiAffiliated withBotany Department, Jomo Kenyatta University of Agriculture and Technology
  • , Ming-Quan ZhouAffiliated withLotus Center, Wuhan University Email author 
  • , Zhong-Li HuAffiliated withKey Lab of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University Email author 
  • , Yun-Chun SongAffiliated withKey Lab of the Ministry of Education for Plant Developmental Biology, College of Life Science, Wuhan University

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Abstract

In this study, outcrossing rates and genetic diversity in natural populations of Nelumbo nucifera were investigated. The estimated multilocus outcrossing rate (tm) based on 28 ISSR loci was over 90%. Analysis of genetic diversity revealed that this index was high at the species level (Hs = 0.325, I = 0.514), but low within the individual study populations (Hs = 0.148, I = 0.212). Gst-B was 0.547 and Nm was 0.414. The results of AMOVA indicated that 54.6% of the variation was due to the difference between the regions and 45.4% to the variation within the region. Although the populations were predominantly outcrossing, most of the genetic diversity was attributed to geographical effects instead of their habitats because low sexual recruitment and clonal growth deeply reduced the genetic diversity within the populations. On the basis of the high tm, Gst-B and low Nm values, we recommended that any future conservation plans should include both in situ conservation and germplasm collection.

Keywords

Genetic diversity Genetic structure ISSR Outcrossing rate Nelumbo nucifera