Genome size for the species of Nerine Herb. (Amaryllidaceae) and its evident correlation with growth cycle, leaf width and other morphological characters
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Nuclear DNA content (2C) is used as a new criterion to investigate nearly all species of the genus Nerine Herb. The species have the same chromosome number (2n = 2x = 22), with the exception of three triploid plants found. The nuclear DNA content of the diploids, as measured by flow cytometry with propidium iodide, is demonstrated to range from 18.0–35.3 pg. This implies that the largest genome contains roughly 2 × 1010 more base pairs than the smallest. The species, arranged according to increasing genome size, fell apart in three groups if growth cycle and leaf width were also considered. A narrow-leafed, evergreen group with a DNA content between 18.0 and 24.6 pg contains thirteen species, a broad-leaved winter growing group with four species has a DNA content from 25.3–26.2 pg and a broad-leafed summer growing group has a DNA content of 26.8–35.3 pg and contains six species. If the presence of filament appendages and hairiness of the pedicels were also considered, the thirteen evergreen species could be further divided into a group without filament appendages or hairy pedicels with a DNA content of 18.0–18.7 pg. A second group without filament appendages but with hairy pedicels had a DNA content of 19.7–22.3 pg. And a third group with both filament appendages and hairy pedicels had a DNA content of 22.0–24.6 pg. The exception is N. marincowitzii that, despite a low DNA content and narrow leaves is summer growing. The broad-leafed group is further characterised by the absence of filament appendages and the absence of strongly hairy pedicels. The exception here is N. pusilla that, despite a high DNA content, has narrow leaves and minutely hairy pedicels. Nuclear DNA content as measured by flow cytometry is shown to be relevant to throw new light on the relationships between Nerine species.
- Baker, J. G. (1896) Nerine duparquetiana. Flora capensis 6: pp. 214
- Bennett, M. D. (1972) Nuclear DNA content and minimum generation time in herbaceous plants. Proc. Roy. Soc. London 181: pp. 109-135
- Bolus L. (1938) Nerine hesseoides. The Flowering Plants of South Africa 18 t. 683.
- Duncan G. D. (2002a) Grow Nerines. Kirstenbosch Gardening Series. Nat. Bot. Inst., Kirstenbosch, RSA.
- Duncan G. D. (2002b) Nerine gaberonensis. Curtis’s Botanical Magazine 19 (3): 173–177.
- Duncan, G. D. (2002c) The genus Nerine. Bulbs 4: pp. 9-15
- Dyer R. A. (1951) Nerine duparquetiana. The Flowering Plants of Africa 28 t. 1118.
- Dyer R. A. (1952) Nerine huttoniae. The Flowering Plants of Africa 29 t. 1130.
- Gouws, J. B. (1949) Karyology of some South African Amaryllidaceae. Plant Life 5: pp. 54-60
- Greilhuber, J. (1979) Evolutionary changes of DNA and heterochromatin amounts in the Scilla bifolia Group (Liliaceae). Pl. Syst. Evol. Suppl 2: pp. 263-280
- Greilhuber, J. (1998) Intraspecific variation in genome size: a critical reassessment. Ann. Bot 82: pp. 27-35 CrossRef
- Greilhuber, J., Dolezel, J., Lysak, M. A., Bennett, M. D. (2005) The origin, evaluation and proposed stabilisation of the terms `Genome size' and `C-value' to describe nuclear DNA contents. Ann. Bot 95: pp. 255-260
- Grime, J. P. (1998) Plant classification for ecological purposes: is there a role for genome size?. Ann. Bot 82: pp. 117-120
- Johnston, J. S., Bennett, M. D., Rayburn, A. L., Galbraith, D. W., Price, H. J. (1999) Reference standards for determination of DNA content of plant nuclei. Amer. J. Bot 86-5: pp. 609-613
- Kalender, R., Tanskanen, J., Immonen, S., Nevo, E., Schulman, A. H. (2000) Genome evolution of wild barley (Hordeum spontaneum) by Bare-1 retrotransposon dynamics in response to sharp microclimatic divergence. Proc. Natl. Acad. USA 97: pp. 6603-6607
- Norris, C.A. (1974) The genus Nerine. Part II Bull. of the Nerine Soc 6: pp. 7-31
- Ohri, D. (1998) Genome size variation and plant systematics. Ann. Bot. (London) 82: pp. 75-83
- Obermeyer, A. A. Nerine. In: Arnold, T. H., Wet, B. C. eds. (1993) Plants of Southern Africa, names and distribution: 162. National Botanical Institute, Pretoria
- Schönland, S. (1903) New and little known plants. Records of the Albany Museum 1: pp. 49
- Snijman, D. A. (1995) A new Nerine species (Amaryllidaceae, tribe Amaryllidae) from the Koup Karroo, South Africa. Novon 5: pp. 103-105
- Tiersch, T. R., Chandler, R. W., Wachtel, S. S. M., Ellias, S. (1989) Reference standards for flow cytometry and application in comparative studies of nuclear DNA content. Cytometry 10: pp. 706-710 CrossRef
- Traub, H. P. (1967) Review of the genus Nerine Herb. Plant Life 23: pp. 3-32
- Zonneveld, B. J. M. (2001) Nuclear DNA contents of all species of Helleborus discriminate between species and sectional divisions. Pl. Syst. Evol 229: pp. 125-130 CrossRef
- Zonneveld, B. J. M., Iren, F. (2001) Genome size and pollen viability as taxonomic criteria: Application to the genus Hosta. Pl. Biol 3: pp. 176-185
- Zonneveld, B. J. M., Grimshaw, J. M., Davis, A. P. (2003) The systematic value of nuclear DNA content in Galanthus. Pl. Syst. Evol 241: pp. 89-102
- Zonneveld, B. J. M., Duncan, G. D. (2003) Taxonomic implications of genome size and pollen colour and vitality for species of Agapanthus L'Heritier (Agapanthaceae). Pl. Syst. Evol 241: pp. 115-123
- Zonneveld, B. J. M. (2003) The systematic value of nuclear DNA content in Clivia. Herbertia 57: pp. 41-47
- Zonneveld, B. J. M., Jaarsveld, E. J. (2005) Taxonomic implications of genome size for all species of the genus Gasteria Duval (Aloaceae). Pl. Syst. Evol 251: pp. 217-227 CrossRef
- Genome size for the species of Nerine Herb. (Amaryllidaceae) and its evident correlation with growth cycle, leaf width and other morphological characters
Plant Systematics and Evolution
Volume 257, Issue 3-4 , pp 251-260
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- DNA content
- genome size
- flow cytometry
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- Author Affiliations
- 1. Institute of Biology, Leiden University, Clusius Laboratory, Wassenaarse Weg 64, 2333, AL, Leiden, The Netherlands
- 2. South African National Biodiversity Institute, Kirstenbosch Botanical Garden, Private bag X7, Claremont, 7735, Cape Town, South Africa