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Integration of sample pretreatment, μPCR, and detection for a total genetic analysis microsystem

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Abstract

Since the emergence of lab-on-a-chip technology, a variety of chemical and biochemical assays were successfully implemented on microdevice platforms. Among the chip-based applications, genetic analysis based on the polymerase chain reaction (PCR) has been extensively developed in order to accomplish the goal of cheap, rapid, high-throughput, and point-of-care DNA testing. We are summarizing here several formats of the miniaturized PCR systems including the integration of units for sample pretreatment and downstream analytical detection. The various sections cover (a) miniaturized PCR systems, (b) integrated sample pretreatment-PCR microsystems, (c) integrated PCR-detection microsystems, and (d) integrated sample pretreatment-PCR-detection microsystems. Respective microdevices were successfully introduced recently in the form of a fully integrated microsystem for genetic analysis with sample-in-answer-out capability. Contains 120 references.

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Acknowledgment

This work was supported by the Korea CCS R&D Center (2013M1A8A1040878), by the Advanced Biomass R&D Center (ABC) funded by the Ministry of Education, Science and Technology (2011–0031357), and the research project for practical use and advancement of forensic DNA analysis of Supreme Prosecutors’ Office, Republic of Korea (1333304, 2012)

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  1. Department of Chemical and Biomolecular Engineering (BK21 Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Korea

    Byung Hyun Park, Yong Tae Kim, Jae Hwan Jung & Tae Seok Seo

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  1. Byung Hyun Park
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  2. Yong Tae Kim
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  3. Jae Hwan Jung
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  4. Tae Seok Seo
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Correspondence to Tae Seok Seo.

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Park, B.H., Kim, Y.T., Jung, J.H. et al. Integration of sample pretreatment, μPCR, and detection for a total genetic analysis microsystem. Microchim Acta 181, 1655–1668 (2014). https://doi.org/10.1007/s00604-013-1128-y

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  • DOI: https://doi.org/10.1007/s00604-013-1128-y

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