Microchimica Acta

, Volume 180, Issue 15, pp 1517–1522

Protein-directed in situ synthesis of platinum nanoparticles with superior peroxidase-like activity, and their use for photometric determination of hydrogen peroxide

Short Communication

DOI: 10.1007/s00604-013-1068-6

Cite this article as:
Chen, L., Wang, N., Wang, X. et al. Microchim Acta (2013) 180: 1517. doi:10.1007/s00604-013-1068-6


Platinum nanoparticles (Pt-NPs) with sizes in the range from 10 to 30 nm were synthesized using protein-directed one-pot reduction. The model globular protein bovine serum albumin (BSA) was exploited as the template, and the resulting BSA/Pt-NPs were studied by transmission electron microscopy, energy dispersive X-ray spectroscopy, and resonance Rayleigh scattering spectroscopy. The modified nanoparticles display a peroxidase-like activity that was exploited in a rapid method for the colorimetric determination of hydrogen peroxide which can be detected in the 50 μM to 3 mM concentration range. The limit of detection is 7.9 μM, and the lowest concentration that can be visually detected is 200 μM.


Pt-NPs were synthesized using BSA-directed one-pot reduction and BSA/Pt-NPs composite can effectively catalyze the oxidation of TMB producing blue solution in the presence of H2O2.


Bovine serum albuminIn situ synthesisPlatinum nanoparticlesColorimetric detection

Supplementary material

604_2013_1068_MOESM1_ESM.doc (982 kb)
ESM 1(DOC 982 kb)

Copyright information

© Springer-Verlag Wien 2013

Authors and Affiliations

  • Lijian Chen
    • 1
  • Nan Wang
    • 1
  • Xindong Wang
    • 1
  • Shiyun Ai
    • 1
  1. 1.College of Chemistry and Material ScienceShandong Agricultural UniversityTaianPeople’s Republic of China