Microchimica Acta

, Volume 179, Issue 3, pp 265–272

Tin disulfide nanoflakes decorated with gold nanoparticles for direct electrochemistry of glucose oxidase and glucose biosensing

Original Paper

DOI: 10.1007/s00604-012-0889-z

Cite this article as:
Li, J., Yang, Z., Zhang, Y. et al. Microchim Acta (2012) 179: 265. doi:10.1007/s00604-012-0889-z


Previously, we have prepared nanoflake-like tin disulfide (SnS2) and used for the immobilization of proteins and biosensing. We have now modified an electrode with a composite consisting of nanoflake-like SnS2 decorated with gold nanoparticles (Au-NPs) and have immobilized glucose oxidase (GOx) on its surface in order to study its direct electrochemistry. Scanning electron microscopy, electrochemical impedance spectroscopy, Fourier transform IR spectroscopy and cyclic voltammetry were used to examine the interaction between GOx and the AuNP-SnS2 film. It is shown that the composite film has a larger surface area and offers a microenvironment that facilitates the direct electron transfer between enzyme and electrode surface. The immobilized enzyme retains its bioactivity and undergoes a surface-controlled, reversible 2-proton and 2-electron transfer reaction, with an apparent electron transfer rate constant of 3.87 s -1. Compared to the nanoflake-like SnS2-based glucose sensor, the GOx-based biosensor exhibits a lower detection limit (1.0 :M), a better sensitivity (21.8 mA M -1  cm -2), and a wider linear range (from 0.02 to 1.3 mM). The sensor displays excellent selectivity, good reproducibility, and acceptable stability. It was successfully applied to reagentless sensing of glucose at −0.43 V.


The AuNPs decorated nanoflake-like SnS2 (AuNPs–SnS2) composite is for the first time prepared and used to construct novel glucose biosensor nanoflake-like SnS2 was firstly synthesized and SEM image of the nanoflake-like SnS2 (a) and TEM images of the nanoflake-like SnS2 (b), AuNPs (c) and AuNPs–SnS2 (d) are shown in above figure.


Nanoflake-like SnS2Au nanoparticlesGlucose oxidaseDirect electrochemistryBiosensor

Supplementary material

604_2012_889_MOESM1_ESM.doc (160 kb)
Esm 1(DOC 160 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  1. 1.College of Chemistry and Chemical EngineeringYangzhou UniversityYangzhouPeople’s Republic of China