Microchimica Acta

, Volume 173, Issue 3, pp 347–352

Sensitive DNA-hybridization biosensors based on gold nanoparticles for testing DNA damage by Cd(II) ions

Original Paper

DOI: 10.1007/s00604-011-0558-7

Cite this article as:
Zhang, Q., Dai, P. & Yang, Z. Microchim Acta (2011) 173: 347. doi:10.1007/s00604-011-0558-7

Abstract

A DNA biosensor was constructed by immobilizing a 20-mer oligonucleotide probe and hybridizing it with its complementary oligomer on the surface of a glassy carbon electrode modified with gold nanoparticles. The properties of the biosensor and its capability of recognizing its complementary sequence were studied by electrochemical impedance spectroscopy. The oxidative stress caused by cadmium ions can be monitored by differential pulse voltammetry using the cobalt(III)tris(1,10-phenanthroline) complex and methylene blue as electrochemical indicators. The biosensor is capable of indicating damage caused by Cd(II) ions in pH 6.0 solution. The results showed that the biosensor can be used for rapid screening for DNA damage.

Figure

DPV of DNA biosensors before (a, c) and after hybridization (b, d) at 1.0 ×10–7 mol·L-1target DNA concentration, (a) probe DNA/Au/GCE and (b) dsDNA/Au/GCE (c) probe DNA/GCE, (d) dsDNA/GCE

Keywords

DNA-hybridization biosensor DNA damage Cd(II) ion Co(phen)33+ Methylene blue 

Supplementary material

604_2011_558_MOESM1_ESM.doc (554 kb)
Fig. S1Nyquist diagrams recorded at the probe DNA/Au/GCE (a) and after hybridization reaction with different concentrations of sequence- specific DNA of PAT gene: (b) 1.0 × 10−12 mol.L−1, (c) 1.0 × 10−11 mol.L−1, (d) 1.0 × 10−10 mol.L−1, (e) 1.0 × 10−9 mol.L−1, (f) 1.0 × 10−8 mol.L−1, (g) 1.0 × 10−7 mol.L−1 and (h) 1.0 × 10−6 mol.L−1. (DOC 554 kb)
604_2011_558_MOESM2_ESM.doc (475 kb)
Fig. S2Nyquist diagrams recorded of dsDNA/nano- Au/GCE in 5.0 mmol.L−1 pH 6.0 Tris–HCl buffer solution after damaged by 0.02 mmol.L−1 Cd2+ for different time: 0 min, 10 min, 20 min, 30 min,50 min (a→e). (DOC 475 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  1. 1.Anhui Key Laboratory of Chemo-Biosening, College of Chemistry and Materials ScienceAnhui Normal UniversityWuhuPeople’s Republic of China