Microchimica Acta

, Volume 173, Issue 1, pp 189–197

Microfluidic robotic device coupled with electrochemical sensor field for handling of paramagnetic micro-particles as a tool for determination of plant mRNA

  • Dalibor Huska
  • Vojtech Adam
  • Petr Babula
  • Libuse Trnkova
  • Jaromir Hubalek
  • Josef Zehnalek
  • Ladislav Havel
  • Rene Kizek
Original Paper

DOI: 10.1007/s00604-011-0545-z

Cite this article as:
Huska, D., Adam, V., Babula, P. et al. Microchim Acta (2011) 173: 189. doi:10.1007/s00604-011-0545-z

Abstract

The expression of genes responsible for the biosynthesis of stress proteins corresponds to the exposition of an organism to abiotic and/or biotic stress. We utilize two types of paramagnetic particles for isolation of total mRNA from early somatic embryos of Norway Spruce (Picea abies /L./ Karst.) and maize plants (Zea mays L.) treated with cadmium(II) ions. The paramagnetic particles were evaluated for analysis of real samples, and poly-adenine was used as a model mRNA. Various approaches (from non-automatic to fully automatic) were tested in terms of handling the particles.

Figa

Microfluidic robotic device coupled with electrochemical sensor field

Keywords

Electrochemical detection Microfluidics Environmental stress Cadmium ions Paramagnetic particles 

Supplementary material

604_2011_545_MOESM1_ESM.doc (3 mb)
ESM 1(DOC 3080 kb)

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Dalibor Huska
    • 1
    • 2
  • Vojtech Adam
    • 1
  • Petr Babula
    • 3
  • Libuse Trnkova
    • 4
  • Jaromir Hubalek
    • 5
  • Josef Zehnalek
    • 1
  • Ladislav Havel
    • 2
  • Rene Kizek
    • 1
  1. 1.Department of Chemistry and BiochemistryMendel UniversityBrnoCzech Republic
  2. 2.Department of Plant BiologyMendel UniversityBrnoCzech Republic
  3. 3.Department of Natural Drugs, Faculty of PharmacyUniversity of Veterinary and Pharmaceutical SciencesBrnoCzech Republic
  4. 4.Department of Chemistry, Faculty of ScienceMasaryk UniversityBrnoCzech Republic
  5. 5.Department of Microelectronics, Faculty of Electrical Engineering and CommunicationBrno University of TechnologyBrnoCzech Republic