, Volume 171, Issue 3-4, pp 437-440

In vitro monitoring of picogram levels of risperidone in human urine via luminollysozyme flow injection chemiluminescence

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Abstract

The anti-schizophrenic drug risperidone (RSP) exerts an inhibitory effect on the chemiluminescence (CL) of the luminol-lysozyme system. This finding forms the basis for a sensitive flow injection method for its determination at picogram levels. RSP binds to Trp62 in the lysozyme, and this leads to a conformational change upon which the CL of the system is quenched. The decrease in CL is proportional to the logarithm of the concentration of RSP, and the calibration graph is linear in the range from 0.1 pg mL−1 to 1.0 ng mL−1, with relative standard deviations of <5.0%, and a detection limit of 0.05 pg mL−1 (3σ). At a flow rate of 2.0 mL min−1, the whole process including sampling and washing is completed within 20 s. The method was successfully applied to monitoring RSP in human urine after incorporation of 2 mg of RSP, with a total excretion of 16.6% within 8.5 h.

Figure

The reaction of lysozyme with risperidone using luminol as luminescence reagent by the luminol-lysozyme FI-CL system and its application.