Microchimica Acta

, Volume 162, Issue 1, pp 261–268

Biosensor based on horseradish peroxidase modified carbon nanotubes for determination of 2,4-dichlorophenol

Authors

    • Life and Environmental Science CollegeShanghai Normal University
  • Yongxia Qu
    • Life and Environmental Science CollegeShanghai Normal University
  • Ruina Li
    • Life and Environmental Science CollegeShanghai Normal University
  • Jian Shen
    • Life and Environmental Science CollegeShanghai Normal University
  • Liwei Zhu
    • Life and Environmental Science CollegeShanghai Normal University
Original Paper

DOI: 10.1007/s00604-007-0872-2

Cite this article as:
Huang, S., Qu, Y., Li, R. et al. Microchim Acta (2008) 162: 261. doi:10.1007/s00604-007-0872-2

Abstract.

A novel and convenient strategy is presented for determination of 2,4-dichlorophenol (2,4-DCP). Horseradish peroxidase (HRP) was self-assembled on a multiwalled carbon nanotubues (MWNTs) modified glassy carbon (GC) electrode. In the presence of hydrogen peroxide (H2O2), 2,4-DCP can be oxidized at this enzyme electrode and the reduction current is proportional to the concentration of the 2,4-DCP. The method showed good linearly for 1.0 × 10−6–1.0 × 10−4 M 2,4-DCP with a detection limit of 3.8 × 10−7 M under the optimal conditions. The peak current of the HRP-MWNTs-GC electrode decreased by about 15% over two weeks. The mechanism of the enzyme biosensor was also studied, and a kinetic equation was derived. The performance of the electrode was verified by determination of 2,4-DCP in environmental water.

Keywords: 2,4-Dichlorophenol; carbon nanotubules; horseradish peroxidase; detection; kinetic equation

Copyright information

© Springer-Verlag 2007