A simple method to fabricate a Prussian Blue nanoparticles/carbon nanotubes/poly(1,2-diaminobenzene) based glucose biosensor Original Paper First Online: 31 July 2007 Received: 18 March 2007 Accepted: 05 June 2007 DOI:
Cite this article as: Zeng, J., Wei, W., Liu, X. et al. Microchim Acta (2008) 160: 261. doi:10.1007/s00604-007-0818-8 Abstract.
A novel Prussian Blue nanoparticles/carbon nanotubes/poly(1,2-diaminobenzene) based glucose biosensor was fabricated by a simple and fast method. Firstly, the Prussian Blue (PB) nanoparticles were direct electrodeposited onto the surface of a glassy carbon electrode modified with multiwall carbon nanotubes (MWNTs) in a short time. Then an ultrathin conducting poly(1,2-diaminobenzene) film was electrodeposited onto the surface of PB/MWNTs nanocomposite to immobilize the glucose oxidase via glutaraldehyde cross-linking. The experiments results showed that the stability of the PB nanoparticles was greatly improved by the MWNTs and the PB/MWNTs nanocomposite exhibited an excellent synergistic electrocatalytic effect toward reduction of hydrogen peroxide. The biosensor showed excellent performances toward determination of glucose. The linear range of the glucose biosensor is from 10 µM to 2.5 mM, with a detection limit of about 5 µM. The response is within less than 5 sec.
Keywords: Prussian Blue; carbon nanotubes; nanocomposite; electrodeposition; glucose; biosensors
Correspondence: Wanzhi Wei, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Hunan, Changsha 410082, P.R. China
References Iijima, S 1991 Helical microtubules of graphitic carbon Nature 354 56 CrossRef Google Scholar Ebbesen, T W 1997Carbon nanotubes: preparation and properties CRC Press Boca Raton, FL 111 Google Scholar Baughman, R H, Zakhidov, A, Heer, W A D 2002 Carbon nanotubes-the route toward applications Science 297 787 CrossRef Google Scholar Terrones, M 2003 Science and technology of the twenty-first century: synthesis, properties, and applications of carbon nanotubes Annu Rev Mater Res 33 419 CrossRef Google Scholar Ajayan, P M 1999 Nanotubes from carbon Chem Rev 99 1787 CrossRef Google Scholar Wang, J, Liu, G, Jan, M R 2004 Ultrasensitive electrical biosensing of proteins and DNA: carbon-nanotube derived amplification of the recognition and transduction events J Am Chem Soc 126 3010 CrossRef Google Scholar Gao, X H, Wei, W Z, Yang, L, Guo, M L 2006 Carbon nanotubes/poly(1,2-diaminobenzene) nanoporous composite film electrode prepared by multipulse potentiostatic electropolymerisation and its application to determination of trace heavy metal ions Electroanalysis 18 485 CrossRef Google Scholar Zhang, M G, Gorski, W 2005 Electrochemical sensing platform based on the carbon nanotubes/redox mediators-biopolymer system J Am Chem Soc 127 2058 CrossRef Google Scholar Zhang, M G, Smith, A, Gorski, W 2004 Electrochemical sensing based on redox mediation at carbon nanotubes Anal Chem 76 5045 CrossRef Google Scholar Yang, M H, Yang, Y H, Liu, Y L, Shen, G L, Yu, R Q 2006 Platinum nanoparticles-doped sol-gel/carbon nanotubes composite electrochemical sensors and biosensors Biosens Bioelectron 21 1125 CrossRef Google Scholar Yang, M H, Jian, J H, Yang, Y H, Chen, X H, Shen, G L, Yu, R Q 2006 Carbon nanotube/cobalt hexacyanoferrate nanoparticle-biopolymer system for the fabrication of biosensors Biosens Bioelectron 21 1791 CrossRef Google Scholar Yang, M H, Yang, Y H, Qu, F L, Lu, Y S, Shen, G L, Yu, R Q 2006 Attachment of nickel hexacyanoferrates nanoparticles on carbon nanotubes: preparation, characterization and bioapplication Anal Chim Acta 571 211 CrossRef Google Scholar Zhang, X T, Zhang, J 2005 Conducting polymer/carbon nanotube composite films made by in situ electropolymerization using an ionic surfactant as the supporting electrolyte Carbon 43 2186 CrossRef Google Scholar Ricci, F, Palleschi, G 2005 Sensor and biosensor preparation, optimisation and applications of prussian blue modified electrodes Biosen Bioelectron 21 389 CrossRef Google Scholar Koncki, R, Wolfbeis Otto, S 1999 Composite films of Prussian blue and N-substituted polypyrroles: covalent immobilization of enzymes and application to near infrared optical biosensing Biosen Bioelectron 14 87 CrossRef Google Scholar Moscone, D, D’Ottavi, D, Compagnone, D, Palleschi, G, Amine, A 2001 Construction and analytical characterization of Prussian Blue-based carbon paste electrodes and their assembly as oxidase enzyme sensors Anal Chem 73 2529 CrossRef Google Scholar Karyakin, A, Guitelmaker, O, Karyakina, E 1995 Prussian blue-based first-generation biosensor: a sensitive amperometric electrode for glucose Anal Chem 67 2419 CrossRef Google Scholar Karyakin, A, Karyakina, E 1999 Prussian blue-based “artificial peroxidase” as a transducer for hydrogen peroxide detection: application to biosensors Sens Actuators B 57 168 CrossRef Google Scholar Karyakin, A, Karyakina, E, Gorton, L 2000 Amperometric biosensor for glutamate using prussian blue-based “Artificial Peroxidase” as a transducer for hydrogen peroxide Anal Chem 72 1720 CrossRef Google Scholar Guo, Y Z, Guadalupe Ana, R 1999 Chemically derived prussian blue sol-gel composite thin films Chem Mater 11 135 CrossRef Google Scholar Zhang, D, Wang, K, Sun, D C, Xia, X H, Chen, H Y 2003 Ultrathin layers of densely packed prussian blue nanoclusters prepared from a ferricyanide solution Chem Mater 15 4163 CrossRef Google Scholar Zhai, X R, Wei, W Z, Zeng, J X, Liu, X Y, Gong, S G 2006 New nanocomposite based on prussian blue nanoparticles/carbon nanotubes/chitosan and its application for assembling of amperometric glucose biosensor Anal Lett 39 913 CrossRef Google Scholar Zhu, L D, Zhai, J L, Guo, Y N, Tian, C Y, Yang, R L 2006 Amperometric glucose biosensors based on integration of glucose oxidase onto prussian blue/carbon nanotubes nanocomposite electrodes Electroanalysis 18 1842 CrossRef Google Scholar Zhang, Y J, Wen, Y, Liu, Y, Li, D, Li, J H 2004 Functionalization of single-walled carbon nanotubes with Prussian blue Electrochem Commu 6 1180 CrossRef Google Scholar Qian, L, Yang, X R 2006 Assembly of prussian blue onto SiO 2 nanoparticles and carbon nanotubes by electrostatic interaction Colloid Surfaces A Physicochem Eng Aspects 278 123 CrossRef Google Scholar Li, Z F, Chen, J H, Li, W, Chen, K, Nie, L H, Yao, S Z 2007 Improved electrochemical properties of prussian blue by multi-walled carbon nanotubes J Electroanal Chem 603 59 CrossRef Google Scholar Curulli, A, Valentini, F, Orlanduci, S, Terranova, M L, Palleschi, G 2004 Pt based enzyme electrode probes assembled with prussian blue and conducting polymer nanostructures Biosen Bioelectron 20 1223 CrossRef Google Scholar Kim, B, Sigmund, W M 2004 Functionalized multiwall carbon nanotube/gold nanoparticle composites Langmuir 20 8239 CrossRef Google Scholar Pan, D W, Chen, J H, Nie, L H, Tao, W Y, Yao, S Z 2004 Amperometric glucose biosensor based on immobilization of glucose oxidase in electropolymerized o-aminophenol film at Prussian blue-modified platinum electrode Electrochim Acta 49 795 CrossRef Google Scholar Luo, H, Shi, Z, Li, N, Gu, Z, Zhuang, Q 2001 Investigation of the electrochemical and electrocatalytic behavior of single-wall carbon nanotube film on a glassy carbon electrode Anal Chem 73 915 CrossRef Google Scholar Gong, K, Zhu, X, Zhao, R, Xiong, S, Mao, L, Chen, C 2005 Rational attachment of synthetic triptycene orthoquinone onto carbon nanotubes for electrocatalysis and sensitive detection of thiols Anal Chem 77 8158 CrossRef Google Scholar Lei, S, Gao, F, Mao, L 2006 Electrochemical properties of carbon nanotube (CNT) film electrodes prepared by controllable adsorption of CNTs onto an alkanethiol monolayer self-assembled on gold electrodes Anal Chem 78 2651 CrossRef Google Scholar Karyakin, A A, Gitelmacher, O V 1995 Prussian blue-based first-generation biosensor: a sensitive amperometric electrode for glucose Anal Chem 67 2419 CrossRef Google Scholar Chi, Q J, Dong, S J 1995 Amperometric biosensors based on the immobilization of oxidases in a prussian blue film by electrochemical codeposition Anal Chim Acta 310 429 CrossRef Google Scholar Lin, Y, Lu, F 2004 Glucose biosensors based on carbon nanotube nanoelectrode ensembles Nano Lett 4 192 Google Scholar