Microchimica Acta

, Volume 160, Issue 1, pp 261–267

A simple method to fabricate a Prussian Blue nanoparticles/carbon nanotubes/poly(1,2-diaminobenzene) based glucose biosensor

  • Jinxiang Zeng
  • Wanzhi Wei
  • Xiaoyin Liu
  • Ying Wang
  • Guangming Luo
Original Paper

DOI: 10.1007/s00604-007-0818-8

Cite this article as:
Zeng, J., Wei, W., Liu, X. et al. Microchim Acta (2008) 160: 261. doi:10.1007/s00604-007-0818-8

Abstract.

A novel Prussian Blue nanoparticles/carbon nanotubes/poly(1,2-diaminobenzene) based glucose biosensor was fabricated by a simple and fast method. Firstly, the Prussian Blue (PB) nanoparticles were direct electrodeposited onto the surface of a glassy carbon electrode modified with multiwall carbon nanotubes (MWNTs) in a short time. Then an ultrathin conducting poly(1,2-diaminobenzene) film was electrodeposited onto the surface of PB/MWNTs nanocomposite to immobilize the glucose oxidase via glutaraldehyde cross-linking. The experiments results showed that the stability of the PB nanoparticles was greatly improved by the MWNTs and the PB/MWNTs nanocomposite exhibited an excellent synergistic electrocatalytic effect toward reduction of hydrogen peroxide. The biosensor showed excellent performances toward determination of glucose. The linear range of the glucose biosensor is from 10 µM to 2.5 mM, with a detection limit of about 5 µM. The response is within less than 5 sec.

Keywords: Prussian Blue; carbon nanotubes; nanocomposite; electrodeposition; glucose; biosensors

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Jinxiang Zeng
    • 1
    • 2
  • Wanzhi Wei
    • 1
  • Xiaoyin Liu
    • 1
  • Ying Wang
    • 1
  • Guangming Luo
    • 2
  1. 1.State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical EngineeringHunan UniversityHunanP.R. China
  2. 2.Jiangxi Province Chinese Native Medicine Idioplasm Resources Project Engineering Research CenterJiangxi University of Traditional Chinese MedicineJiangxiP.R. China