Microchimica Acta

, Volume 153, Issue 1, pp 15–20

PNA-Modified Magnetic Nanoparticles and Their Hybridization with Single-Stranded DNA Target: Surface Enhanced Raman Scatterings Study


DOI: 10.1007/s00604-005-0460-2

Cite this article as:
Wang, F., Shen, H., Feng, J. et al. Microchim Acta (2006) 153: 15. doi:10.1007/s00604-005-0460-2


A 4-pyridyldithiol-derivatized peptide nucleic acid (PNA), which was designed to recognize a specific gene, was attached to 3-mercapropropyloxysilane coated magnetic nanoparticles (MNPs) via a thiol-disulfide exchange reaction. Subsequently, PNA-functionalized magnetic nanoparticles (PMNPs) were prepared. Sequentially the PMNPs were challenged with non-complementary and perfect-match DNA targets. The entire procedure was monitored using surface-enhanced Raman scattering (SERS). The results showed that the PMNPs were able to efficiently hybridize with the perfect-match ssDNA target and showed no affinity towards non-complementary DNA. This approach may provide a means of direct and label-free gene analysis.

Key words: Peptide nucleic acid (PNA); magnetic nanoparticles (MNPs); PNA-modified MNPs (PMNPs); surface-enhanced Raman scattering (SERS).

Copyright information

© Springer-Verlag/Wien 2005

Authors and Affiliations

  1. 1.College of Life and Environment Science, Shanghai Normal UniversityShanghaiChina