Microchimica Acta

, Volume 148, Issue 3, pp 335–341

Electropolymerization of Azure B on a Screen-Printed Carbon Electrode and its Application to the Determination of NADH in a Flow Injection Analysis System


DOI: 10.1007/s00604-004-0283-6

Cite this article as:
Sha, Y., Gao, Q., Qi, B. et al. Microchim. Acta (2004) 148: 335. doi:10.1007/s00604-004-0283-6


The electrooxidation polymerization of azure B on screen-printed carbon electrodes in neutral phosphate buffer was studied. The poly(azure B) modified electrodes exhibited excellent electrocatalysis and stability for dihydronicotinamide adenine dinucleotide (NADH) oxidation in phosphate buffer (pH 6.9), with an overpotential of more than 400 mV lower than that at the bare electrodes. Different techniques, including cyclic voltammetry, scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy have been employed to characterize the poly (azure B) film. Furthermore, the modified screen-printed carbon electrodes were found to be promising as an amperometric detector for the flow injection analysis (FIA) of NADH, typically with a dynamic range of 0.5 µM to 100 µM.

Key words: Electropolymerization; azure B; NADH; screen-printed electrode; FIA.

Copyright information

© Springer-Verlag/Wien 2004

Authors and Affiliations

  1. 1.State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of SciencesChangchun, JilinChina