Microchimica Acta

, Volume 146, Issue 1, pp 13–19

Application of L-Cysteine-Capped ZnS Nanoparticles in the Determination of Nucleic Acids Using the Resonance Light Scattering Method

Authors

    • College of Chemistry and Materials Science, Anhui Normal University
  • Jinlong Chen
    • College of Chemistry and Materials Science, Anhui Normal University
  • Shujuan Zhuo
    • College of Chemistry and Materials Science, Anhui Normal University
  • Yuqin Wu
    • College of Chemistry and Materials Science, Anhui Normal University
  • Changqin Zhu
    • College of Chemistry and Materials Science, Anhui Normal University
  • Lun Wang
    • College of Chemistry and Materials Science, Anhui Normal University
Article

DOI: 10.1007/s00604-003-0168-0

Cite this article as:
Li, Y., Chen, J., Zhuo, S. et al. Microchim. Acta (2004) 146: 13. doi:10.1007/s00604-003-0168-0

Abstract.

Nanometer-sized L-cysteine-capped ZnS particles were synthesized by a colloidal aqueous method. The functionalized nanoparticles are water-soluble and suitable for biological applications. In Tris-HCl buffer solution, nucleic acids combine with cysteine-capped nano-ZnS particles by intermolecular forces to form larger nanoparticles. There are two resonance light scattering peaks at 304.5 nm and 373.8 nm, respectively. The enhanced RLS is related to the concentration of nucleic acids in the range of 0.04 to 1.2 µg mL−1 for calf thymus DNA and 0.2 to 1.0 µg mL−1 for fish sperm DNA. The detection limits (3δ) are 19 ng mL−1 for calf thymus DNA and 23 ng mL−1 for fish sperm DNA, respectively. Four synthetic samples were analyzed satisfactorily.

Key words: Resonance light scattering; nucleic acid; ZnS; cysteine; nanoparticles.
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Copyright information

© Springer-Verlag/Wien 2004