High background levels compromise the use of cardiac troponin I RNA detection in peripheral blood as a diagnostic tool in cardiology
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The detection of cardiac troponins in peripheral blood as protein markers of myocardial infarction is a new diagnostic tool in the diagnosis of cardiac disease. In order to increase the sensitivity and specificity of this diagnostic approach, a reverse transcription polymerase chain reaction assay has been developed to detect the mRNA encoding cardiac troponin I from myocardial cells hypothetically released from damaged cardiac tissue. The detection is specific for cardiac troponin I mRNA, with no amplification of homologous sequences of other troponin I isoforms, i.e., troponin I from skeletal muscle cells. However, a strong amplification signal for cardiac troponin I mRNA was detected in samples of peripheral blood from healthy human volunteers. In patients with acute myocardial infarction or angina pectoris, the cardiac troponin I mRNA levels were not increased over background levels. In conclusion, a reverse transcription polymerase chain reaction approach based on the amplification of cardiac troponin I mRNA is not feasible in the diagnosis of cardiac diseases.
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- High background levels compromise the use of cardiac troponin I RNA detection in peripheral blood as a diagnostic tool in cardiology
International Journal of Clinical and Laboratory Research
Volume 30, Issue 1 , pp 13-15
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Cardiac troponin I
- Peripheral blood
- Background levels
- Industry Sectors
- Author Affiliations
- 1. Department of Clinical Chemistry, Medical Clinic, University Hospital Eppendorf, Martinistrasse 52, 20251, Hamburg, Germany
- 2. Department of Cardiology, Medical Clinic, University Hospital Eppendorf, Martinstrasse 52, 20251, Hamburg, Germany