International Journal of Clinical and Laboratory Research

, 30:187

Cigarette smoking as an aggravating factor in inflammatory tissue-destructive diseases. Increase in tumor necrosis Factor-α priming of peripheral neutrophils measured as generation of oxygen radicals

Authors

  • A. Gustafsson
    • Karolinska Institutet Department of Medical Laboratory Sciences and TechnologyDivision of Clinical Chemistry
    • Karolinska Institutet, Department of OdontologyDivision of Periodontology
  • B. Åsman
    • Karolinska Institutet Department of Medical Laboratory Sciences and TechnologyDivision of Clinical Chemistry
  • K. Bergström
    • Karolinska Institutet Department of Medical Laboratory Sciences and TechnologyDivision of Clinical Chemistry
Original

DOI: 10.1007/s005990070005

Cite this article as:
Gustafsson, A., Åsman, B. & Bergström, K. Int J Clin Lab Res (2000) 30: 187. doi:10.1007/s005990070005

Abstract

Stimulated neutrophils from subjects with various inflammatory tissue-destructive conditions, such as periodontal, pulmonary, gastrointestinal, and cardiovascular diseases, generate more oxygen radicals and proteases, implicated in tissue destruction, than neutrophils from healthy persons. Cigarette smoking aggravates these diseases. The aim of our study was to investigate the effect of cigarette smoking on the priming capacity of tumor necrosis factor-α, measured as generation of radicals from stimulated neutrophils, in smoking and non-smoking subjects with or without periodontitis. The priming effect was higher in neutrophils from smokers. In the group with periodontitis, smoking caused an even greater increase in the generation of radicals, indicating an additive effect of this local disease. The membrane expression of CD11b, CD15, and CD63 was significantly higher on neutrophils from smokers, indicating upregulated neutrophil functions. This increased priming effect of tumor necrosis factor-α in smokers subjects could be of importance in the aggravation of tissue-destructive inflammatory diseases.

Key words

Tumor necrosis factor-α Neutrophils Tissue destruction Cigarette smoking

Copyright information

© Springer-Verlag 2000