Surgery Today

, Volume 36, Issue 12, pp 1085–1093

Protein Pattern Difference in the Colon Cancer Cell Lines Examined by Two-Dimensional Differential In-Gel Electrophoresis and Mass Spectrometry

  • Masafumi Katayama
  • Hiroshi Nakano
  • Atsuko Ishiuchi
  • Wenwen Wu
  • Ryuichi Oshima
  • Joe Sakurai
  • Hiroyuki Nishikawa
  • Susumu Yamaguchi
  • Takehito Otsubo
Article

DOI: 10.1007/s00595-006-3301-y

Cite this article as:
Katayama, M., Nakano, H., Ishiuchi, A. et al. Surg Today (2006) 36: 1085. doi:10.1007/s00595-006-3301-y

Abstract

Purpose

The pivotal metastatic processes of colorectal cancer (CRC) have yet to be fully investigated by a comprehensive all-inclusive protein analysis. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) and liquid chromatography–tandem mass spectrometry (LC/MS/MS) to investigate the protein pattern changes during the metastasis of CRC. Two CRC cell lines were investigated: SW480 derived from the primary lesion and SW620 derived from lymph node metastasis in the same patient.

Methods

The two cell lines were compared using 2D-DIGE with a maleimide CyDye fluorescent protein labeling technique, which has an enhanced sensitivity for many proteins at a low concentration. A comprehensive proteomics analysis was performed by the dual-labeling method using Cy3 and Cy5 and by LC/MS/MS. In addition, an in vivo experiment of metastasis using nude mice was performed by the injection of the two cell lines into the spleen.

Results

Among approximately 1 500 proteins, we detected 9 protein spots with definitively significant changes between the two cell lines. Three out of the nine proteins were validated by a Western blot analysis. Alpha-enolase and triosephosphate isomerase were significantly upregulated in SW620 in comparison to SW480. Annexin A2 (annexin II) was significantly downregulated in SW620 compared to SW480. Neither liver metastasis nor peritoneal dissemination was established in the metastatic experiment using SW480 but some liver and peritoneal metastases occurred in the experiment using SW620. An in vivo metastatic experiment using SW620 showed the expressions of alpha-enolase and triosephosphate isomerase to increase in the liver metastases in comparison to those in the splenic implanted lesion. The expressions of triosephosphate isomerase increased in the peritoneal lesions in comparison to those in the splenic implanted lesion.

Conclusions

2D-DIGE and LC/MS/MS techniques identified nine proteins that increased significantly more in SW620 than in SW480. The finding of our in vivo metastatic experiment suggests that alpha-enolase and triosephosphate isomerase, at least in part, may be associated with the metastatic process of these two cell lines.

Key words

ProteomicsColon cancer cell linesAlpha-enolaseTriosephosphate isomerase

Copyright information

© Springer-Verlag Tokyo 2006

Authors and Affiliations

  • Masafumi Katayama
    • 1
  • Hiroshi Nakano
    • 1
  • Atsuko Ishiuchi
    • 1
  • Wenwen Wu
    • 2
  • Ryuichi Oshima
    • 1
  • Joe Sakurai
    • 1
  • Hiroyuki Nishikawa
    • 2
  • Susumu Yamaguchi
    • 1
  • Takehito Otsubo
    • 1
  1. 1.Division of Gastroenterological SurgerySt. Marianna University HospitalKawasakiJapan
  2. 2.Division of Breast and Endocrine SurgerySt. Marianna University HospitalKawasakiJapan