Acta Diabetologica

, Volume 46, Issue 3, pp 243–248

A fibrin gel carrier system for islet transplantation into kidney subcapsule

  • Jong-Yeon Lim
  • Byoung-Hoon Min
  • Byoung-Geun Kim
  • Hyun-Ju Han
  • Soo-Jin Kim
  • Chan-Wha Kim
  • Sung-Sik Han
  • Jun-Seop Shin
Short Communication

DOI: 10.1007/s00592-008-0073-4

Cite this article as:
Lim, JY., Min, BH., Kim, BG. et al. Acta Diabetol (2009) 46: 243. doi:10.1007/s00592-008-0073-4

Abstract

Islet transplantation is a promising therapeutic option for type 1 diabetes, and actively performed in the clinic as well as in the animal experiments. For the rodent experiments, islet transplantation into kidney subcapsule is widely used to assess islet quality, however, it is often difficult to do using a polyethylene tubing and fine needle because of inherent dead volume of needle and stickiness of the tubing to islets. This problem makes it difficult to interpret the physiological response to different islet doses. Here, we developed a simple fibrin gel carrier system for islet transplantation into kidney subcapsule and utilized it to determine the marginal islet mass sufficient for correction of hyperglycemia in diabetic nude mice.

Keywords

Diabetes Islet transplantation Fibrin gel Marginal mass 

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Jong-Yeon Lim
    • 1
    • 3
  • Byoung-Hoon Min
    • 2
    • 3
  • Byoung-Geun Kim
    • 3
  • Hyun-Ju Han
    • 3
  • Soo-Jin Kim
    • 2
  • Chan-Wha Kim
    • 1
  • Sung-Sik Han
    • 1
  • Jun-Seop Shin
    • 3
  1. 1.Department of Biotechnology, School of Life Sciences and BiotechnologyKorea UniversitySeoulSouth Korea
  2. 2.Department of Life SciencesHallym UniversityKangwon-doSouth Korea
  3. 3.Korea Islet Transplantation Institute, Inc., School of Life Sciences and BiotechnologyKorea UniversitySeoulSouth Korea