European Spine Journal

, 20:353

Titanium and tantalum as mesenchymal stem cell scaffolds for spinal fusion: an in vitro comparative study

  • Juan F. Blanco
  • Fermin M. Sánchez-Guijo
  • Soraya Carrancio
  • Sandra Muntion
  • Jesus García-Briñon
  • Maria-Consuelo del Cañizo
Original Article

DOI: 10.1007/s00586-011-1901-8

Cite this article as:
Blanco, J.F., Sánchez-Guijo, F.M., Carrancio, S. et al. Eur Spine J (2011) 20(Suppl 3): 353. doi:10.1007/s00586-011-1901-8
  • 211 Downloads

Abstract

Introduction

In the last few years, great interest has been focused on tissue engineering as a potential therapeutic approach for musculoskeletal diseases. The role of metallic implants for spinal fusion has been tested in preclinical and clinical settings. Titanium and tantalum have excellent biocompatibility and mechanical properties and are being used in this situation. On the other hand, the therapeutic role of mesenchymal stem cells (MSC) is extensively explored for their multilineage differentiation into osteoblasts.

Objetives

In vitro comparision of titanium and tantalum as MSCs scaffolds.

Material and methods

In the present study, we have compared the in vitro expansion capacity, viability, immunophenotype (both explored by flow cytometry) and multi-differentiation ability of MSC cultured in the presence of either titanium or tantalum fragments. The adherence of MSC to either metal was demonstrated by electron microscopy.

Results

Both metals were able to carry MSC when transferred to new culture flasks. In addition, our study shows that culture of MSC with titanium or tantalum improves cell viability and maintains all their biological properties, with no significant differences regarding the metal employed.

Conclusion

This would support the use of these combinations for clinical purposes, especially in the spinal fusion and reconstruction setting.

Keywords

Titanium scaffold Tantalum scaffold Mesenchymal stem cells Tissue engineering 

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Juan F. Blanco
    • 1
    • 5
  • Fermin M. Sánchez-Guijo
    • 2
    • 5
  • Soraya Carrancio
    • 3
    • 5
  • Sandra Muntion
    • 3
    • 5
  • Jesus García-Briñon
    • 4
    • 5
  • Maria-Consuelo del Cañizo
    • 2
    • 5
  1. 1.Department of Orthopedics, Servicio de Traumatología y COHospital Universitario de SalamancaSalamancaSpain
  2. 2.Department of HematologyHospital Universitario de SalamancaSalamancaSpain
  3. 3.Cell Therapy LabHospital Universitario de SalamancaSalamancaSpain
  4. 4.Department of Cell Biology, Facultad de MedicinaUniversidad de SalamancaSalamancaSpain
  5. 5.Centro en Red de Terapia Celular y Medicina Regenerativa de Castilla y LeónRed Nacional de Terapia Celular (TerCel)SalamancaSpain

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