Original Article

Comparative Clinical Pathology

, Volume 23, Issue 4, pp 1087-1094

First online:

Immunomodulatory effect of Kalpaamruthaa on 7,12-dimethyl benz(a)anthracene-induced mammary carcinoma studied in rats

  • Vijayakanth DharmendraAffiliated withDepartment of Medical Biochemistry, Dr A.L.M. P-G. I.B.M.S., University of Madras
  • , Shanthi PalaniveluAffiliated withDepartment of Pathology, Dr A.L.M. P-G. I.B.M.S., University of Madras
  • , Sachdanandam PanchanadhamAffiliated withDepartment of Medical Biochemistry, Dr A.L.M. P-G. I.B.M.S., University of Madras Email author 

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Abstract

Kapaamruthaa is a modified Siddha preparation prepared in our laboratory. It has been proven to have therapeutic effect against arthritis and liver cancer. The present study was carried out to bring about the protective effect of Kalpaamruthaa on experimental mammary carcinoma in rats with special reference to cytokines, carcinoembryonic antigen (CEA), carbohydrate antigen 15–3 (CA 15–3), immunoglobulin levels, and glycoprotein components. Breast cancer was induced in rats by administering 7,12-dimethylbenz(a)anthracene (DMBA) orally (25 mg/rat) as a single dose. After 90 days of induction, Semecarpus anacardium Linn. (200 mg/kg body weight) and Kalpaamruthaa (KA) (300 mg/kg body weight) were administered for 14 days, by gastric intubation. In mammary carcinoma-bearing rats, the levels of interleukin-6, interleukin-8, and tumor necrosis factor-α were significantly increased (p < 0.001) in serum when compared to control rats. The levels of CEA and CA 15–3 were found to be significantly increased (p < 0.001) in mammary carcinoma-bearing rats. The levels of IgG and IgM were significantly decreased (p < 0.001) whereas IgA levels were significantly increased (p < 0.001) in mammary carcinoma-bearing rats. The levels of glycoprotein components namely hexose, hexosamine, and sialic acid were significantly (p < 0.001) increased in the spleen and thymus of the mammary carcinoma-bearing (group II) rats when compared to control rats. Treatment with KA significantly altered the levels of cytokines, CEA, CA 15–3, immunoglobulins, and glycoprotein components to near normal levels when compared to breast cancer-induced rats. The present study suggests the therapeutic effect of KA in DMBA-induced mammary carcinoma in rats.

Keywords

Kalpaamruthaa DMBA Mammary carcinoma Cytokines Tumor markers Immunoglobulins