, Volume 22, Issue 1, pp 107-112
Date: 13 Dec 2011

The effect of sub-conjunctival platelet-rich plasma in combination with topical acetylcysteine on corneal alkali burn ulcer in rabbits

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Abstract

An experimental study examined the effect of sub-conjunctival platelet-rich plasma (sPRP) in combination with topical acetylcysteine on corneal alkali burn ulcers in rabbits. A total of 20 rabbits were used in this study. After collecting intracardiac blood samples from ten rabbits, platelet-rich plasma was obtained by centrifugation. Alkali wounds were inflicted on the central corneas of rabbits by applying a round filter paper, 6.0 mm in diameter, soaked in 1 M NaOH for 60 s. Only one eye in each rabbit was used. A total of 20 rabbits were allocated into four groups of five animals each. Group 1 served as the control group. Group 2 received 3% N-acetylcysteine (NAC) topically three times daily for 2 weeks. The third group received only sPRP whereas group 4 received sPRP with topical 3% NAC three times daily for 2 weeks. Clinical outcome was monitored by evaluation of epithelial defects, corneal opacity, duration of blepharospasm, corneal vascularisation, duration of ocular discharge and wound area diameter measurement. After 3 weeks eyes were enucleated and corneas were excised for histopathological analysis. Samples were assessed by evaluating the number of epithelial rows, stromal vascularisation and inflammation and stromal collagen arrangement. Comparison between groups showed that group 3 had significantly shorter duration of blepharospasm than the control group. Additionally, group 3 had smaller mean defect area and greater wound healing. Histopathlogical investigation revealed significantly less inflammation and vascularisation in the corneas in group 3; this group also had the best stromal collagen arrangement. In conclusion sPRP seems to improve corneal epithelial burn healing. However, acetylcysteine and sPRP combination may have a retarded healing effect as compared with platelet-rich plasma alone.