Mycorrhiza

, Volume 11, Issue 2, pp 89–94

Rapid molecular typing method for the reliable detection of Asiatic black truffle (Tuber indicum) in commercialized products: fruiting bodies and mycorrhizal seedlings

  • D. Mabru
  • C. Dupré
  • J. P. Douet
  • P. Leroy
  • C. Ravel
  • J. M. Ricard
  • B. Médina
  • M. Castroviejo
  • G. Chevalier
Original Paper

DOI: 10.1007/s005720100103

Cite this article as:
Mabru, D., Dupré, C., Douet, J. et al. Mycorrhiza (2001) 11: 89. doi:10.1007/s005720100103

Abstract 

Although authorized for the French market, the Asiatic black truffle, Tuber indicum, may potentially be sold as T. melanosporum, the ”Perigord black truffle’’, due to difficulties in distinguishing between them by morphological criteria. A molecular method using a DNA extraction kit and specific primers based on the interspecific polymorphism of the internal transcript spacer DNA region has allowed us to amplify T. indicum samples (mycorrhizas, fresh, dried, and frozen ascocarps) and even to detect as little as 1% of Asiatic truffle in a mixture of ground ascocarps or mycorrhizas containing T. melanosporum. Our process is specific, sensitive, convenient and quick, so it could be used for the rapid detection of T. indicum in non-cooked food products and commercialized mycorrhizal seedlings to avoid the ecological damage which would result from its accidental introduction.

Keywords  Tuber indicum Tuber melanosporum Internal transcript spacer sequence analysis Polymerase chain reaction Molecular markers 

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • D. Mabru
    • 1
  • C. Dupré
    • 1
  • J. P. Douet
    • 3
  • P. Leroy
    • 1
  • C. Ravel
    • 1
  • J. M. Ricard
    • 4
  • B. Médina
    • 3
  • M. Castroviejo
    • 2
  • G. Chevalier
    • 1
  1. 1.I.N.R.A., UMR 1095 INRA-UBP Amélioration et santé des plantes, Site de Crouelle, 234 avenue du Brézet, 63039 Clermont-Ferrand Cedex 2, France e-mail: dmabru@clermont.inra.frFR
  2. 2.C.N.R.S., Laboratoire REGER, UMR 5097 bat 3A, 146 rue Léo Saignat, 33077 Bordeaux Cedex, FranceFR
  3. 3.D.G.C.C.R.F., Université de Bordeaux I, 351 Cours de la Libération, 33405 Talence, FranceFR
  4. 4.C.T.I.F.L., Site de Balandran, B.P. 32, 30127 Bellegarde, FranceFR

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