Mycorrhiza

, Volume 8, Issue 4, pp 203–206

Visualization of ribosomal DNA loci in spore interphasic nuclei of glomalean fungi by fluorescence in situ hybridization

  • Sophie Trouvelot
  • Diederik van Tuinen
  • Mohamed Hijri
  • V. Gianinazzi-Pearson
ORIGINAL PAPER

DOI: 10.1007/s005720050235

Cite this article as:
Trouvelot, S., van Tuinen, D., Hijri, M. et al. Mycorrhiza (1999) 8: 203. doi:10.1007/s005720050235

Abstract

 Fluorescence in situ hybridization (FISH) was applied to interphasic nuclei isolated from spores of four species of AM fungi : Scutellospora castanea, Glomus mosseae, Glomus intraradices and Gigaspora rosea. Ribosomal DNA loci were visualized using digoxigenin-labeled 25 S rDNA probes obtained by nested PCR. Several hybridization sites were detected per nucleus and an internuclear variability was observed in the number of loci. This is the first report of successful application of FISH to analyse the genomes of glomalean fungi.

Key words Arbuscular mycorrhizaSpore nucleiFluorescence in situ hybridizationRibosomal DNA loci

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Sophie Trouvelot
    • 1
  • Diederik van Tuinen
    • 1
  • Mohamed Hijri
    • 1
  • V. Gianinazzi-Pearson
    • 1
  1. 1.Laboratoire de Phytoparasitologie INRA/CNRS, Centre de Microbiologie du Sol et de l'Environnement, INRA, BV 1540, F-21034 Dijon Cedex, France e-mail : gianina@epoisses.inra.fr, Fax: +333-80-63-32-63FR