Mycorrhiza

, Volume 24, Issue 4, pp 281–292

Assessment of ectomycorrhizal biodiversity in Tuber macrosporum productive sites

  • Gian Maria Niccolò Benucci
  • Lorenzo Raggi
  • Emidio Albertini
  • Andrea Gógán Csorbai
  • Domizia Donnini
Original Paper

DOI: 10.1007/s00572-013-0538-3

Cite this article as:
Benucci, G.M.N., Raggi, L., Albertini, E. et al. Mycorrhiza (2014) 24: 281. doi:10.1007/s00572-013-0538-3

Abstract

Tuber macrosporum Vittad. is a truffle with superb organoleptic properties, whose cultivation is still in its infancy. For the first time we have aimed to provide information on ectomycorrhizal communities in natural and cultivated T. macrosporum sites. Ectomycorrhizal morphotypes were identified using ITS nrDNA sequencing and sorted into molecular operational taxonomic unit (MOTU). We detected 16 MOTUs in the T. macrosporum cultivated plantation. Ascomycota were the most abundant (86.4 %) with Helvellaceae, Pyronemataceae and Pezizaceae the most common. Twenty-two MOTUs were collected in the natural T. macrosporum site. Basidiomycota morphotypes were plentiful (70.6 %) and Thelephoraceae dominated. Each site had different taxa belowground with only T. macrosporum in common, being more abundant in the natural (18.2 %) than in the cultivated (14.4 %) site. Species richness, Simpson and Shannon diversity indices, taxonomic diversity, distinctness and variation of taxonomic distinctness were lower in the cultivated than in the natural site.

Keywords

Ectomycorrhizal communityTruffle groundMOTUITS sequencingDiversity indices

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Gian Maria Niccolò Benucci
    • 1
  • Lorenzo Raggi
    • 1
  • Emidio Albertini
    • 1
  • Andrea Gógán Csorbai
    • 2
  • Domizia Donnini
    • 1
  1. 1.Department of Applied BiologyUniversity of PerugiaPerugiaItaly
  2. 2.Department of Horticultural TechnologiesSzent István UniversityGödöllőHungary