Mycorrhiza

, Volume 21, Issue 1, pp 17–25

The AD-type ectomycorrhizas, one of the most common morphotypes present in truffle fields, result from fungi belonging to the Trichophaea woolhopeia species complex

Authors

  • Andrea Rubini
    • National Research Council, Plant Genetics Institute, Perugia Division
  • Beatrice Belfiori
    • National Research Council, Plant Genetics Institute, Perugia Division
  • Valentina Passeri
    • National Research Council, Plant Genetics Institute, Perugia Division
  • Leonardo Baciarelli Falini
    • Department of Applied BiologyUniversity of Perugia
  • Sergio Arcioni
    • National Research Council, Plant Genetics Institute, Perugia Division
  • Claudia Riccioni
    • National Research Council, Plant Genetics Institute, Perugia Division
    • National Research Council, Plant Genetics Institute, Perugia Division
Original Paper

DOI: 10.1007/s00572-010-0308-4

Cite this article as:
Rubini, A., Belfiori, B., Passeri, V. et al. Mycorrhiza (2011) 21: 17. doi:10.1007/s00572-010-0308-4

Abstract

Belowground ectomycorrhizal communities are often species rich. Characterization of the ectomycorrhizas (ECMs) underneath native truffle areas and/or cultivation sites is particularly relevant to identifying fungal species that might interfere with or promote truffle propagation and fruiting. Fungal identification at the genus/species level can now be achieved by combining detailed morphological and anatomical descriptions with molecular approaches. In a survey of the mycorrhizal biodiversity of Tuber melanosporum orchards and inoculated host plants in nurseries, we repeatedly sampled ECMs with morphological features resembling those of the ECMs widely known as the AD type. Despite the fact that the AD type is regarded as one of the most competitive fungal species towards Tuber spp., its taxonomical rank has yet to be resolved. By analyzing the 28S and internal transcribed spacer (ITS) rDNA regions, here, we show that AD-type ECMs result from host plant colonization by the pyronemataceous species Trichophaea woolhopeia. Further to this, the 28S and ITS phylogenetic trees built from the AD-type ECMs analyzed sustain the hypothesis that T. woolhopeia is a species complex.

Keywords

EctomycorrhizaTuberMolecular identificationLSUITSTrichophaea woolhopeia

Supplementary material

572_2010_308_MOESM1_ESM.doc (62 kb)
Fig. S1Multiple sequence alignment of the large subunit region. (DOC 61 kb)
572_2010_308_MOESM2_ESM.doc (62 kb)
Fig. S2Multiple sequence alignment of the internal transcribed spacer region. (DOC 61 kb)
572_2010_308_MOESM3_ESM.doc (240 kb)
Fig. S3Neighbor-joining tree based on large subunit sequences from AD-type samples and other Pyronemataceae. Numbers near the branches represent the bootstrap values (1,000 replicates). The sequences from AD-type samples obtained in this study are given in bold. (DOC 240 kb)
572_2010_308_MOESM4_ESM.doc (242 kb)
Fig. S4Maximum-likelihood tree based on large subunit sequences from AD-type samples and other Pyronemataceae. Numbers near the branches represent the bootstrap values (1,000 replicates). The sequences from AD-type samples obtained in this study are given in bold. (DOC 241 kb)

Copyright information

© Springer-Verlag 2010