Mycorrhiza

, Volume 20, Issue 3, pp 209–215

Ectomycorrhizal species associated with Pinus radiata in New Zealand including novel associations determined by molecular analysis

  • Katrin Walbert
  • Tod D. Ramsfield
  • Hayley J. Ridgway
  • E. Eirian Jones
Short Note

DOI: 10.1007/s00572-009-0277-7

Cite this article as:
Walbert, K., Ramsfield, T.D., Ridgway, H.J. et al. Mycorrhiza (2010) 20: 209. doi:10.1007/s00572-009-0277-7

Abstract

Ectomycorrhizal (ECM) associates of the exotic plantation species Pinus radiata were investigated above and below ground over two years in the North Island of New Zealand. ECM species were identified using morphological and molecular (restriction fragment length polymorphism and DNA sequencing) analysis. Eighteen ECM species were observed fruiting above ground; 19 ECM species were identified below ground. In the above ground study, Wilcoxina mikolae, Rhizopogon pseudoroseolus and Inocybe sindonia were noted for the first time as ECM associates of P. radiata in New Zealand. Below ground, the species W. mikolae, R. pseudoroseolus, Rhizopogon luteorubescens, Pseudotomentella sp., Pseudotomentella tristis and Tomentella sp. were found as new associates of P. radiata in New Zealand. Additionally, six ECM types were found that could not be identified with molecular analysis. The putative ECM taxa Tricholoma pessundatum, Laccaria laccata and Hebeloma crustuliniforme were examined by molecular analysis, and species identifications were proposed to be changed to Tricholoma sp., L. laccata and Hebeloma sp. for specimens associated with P. radiata in New Zealand. The species identity of I. sindonia, previously unidentified to species level, was determined with direct sequencing.

Keywords

Ectomycorrhiza Pinus radiata Exotic plantation forest New Zealand RFLP DNA sequence analysis 

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Katrin Walbert
    • 1
    • 2
  • Tod D. Ramsfield
    • 2
  • Hayley J. Ridgway
    • 3
  • E. Eirian Jones
    • 3
  1. 1.Bio-Protection Research CentreLincolnNew Zealand
  2. 2.Forest Protection, ScionRotoruaNew Zealand
  3. 3.Department of Ecology, Faculty of Agriculture and Life SciencesLincoln UniversityLincolnNew Zealand

Personalised recommendations