Mycorrhiza

, Volume 19, Issue 6, pp 393–402

Development and activity of Glomus intraradices as affected by co-existence with Glomus claroideum in one root system

  • Martina Janoušková
  • Pascale Seddas
  • Libor Mrnka
  • Diederik van Tuinen
  • Anna Dvořáčková
  • Marie Tollot
  • Vivienne Gianinazzi-Pearson
  • Miroslav Vosátka
  • Armelle Gollotte
Original Paper

DOI: 10.1007/s00572-009-0243-4

Cite this article as:
Janoušková, M., Seddas, P., Mrnka, L. et al. Mycorrhiza (2009) 19: 393. doi:10.1007/s00572-009-0243-4

Abstract

The co-existence of two arbuscular mycorrhizal fungal (AMF) species, Glomus intraradices and Glomus claroideum, in the root systems of plants was investigated in a greenhouse experiment aimed at reconstructing interactions during an early stage of primary succession on a coal-mine spoil bank in Central Europe. Two plant species, Tripleurospermum inodorum and Calamagrostis epigejos, were inoculated either with one or both AMF species. Fungal development, determined by trypan blue and alkaline phosphatase staining as well as by PCR amplification of rRNA genes with species-specific primers, and the expression of five genes with different metabolic functions in the intraradical structures of G. intraradices were followed after 6 and 9 weeks of cultivation. The two AMF closely co-existed in the root systems of both plants possibly through similar colonisation rates and competitivity. Inoculation with the two fungi, however, did not bring any additional benefit to the host plants in comparison with single inoculation; moreover, plant growth depression observed after inoculation with G. claroideum persisted also in mixed inoculation. The expression of all the assayed G. intraradices genes was affected either by host plant or by co-inoculation with G. claroideum. The effects of both factors depended on the time of sampling, which underlines the importance of addressing this topic in time-course studies.

Keywords

Alkaline phosphataseArbuscular mycorrhizaGene expressionIntraradical myceliumNested PCR

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Martina Janoušková
    • 1
  • Pascale Seddas
    • 2
  • Libor Mrnka
    • 1
  • Diederik van Tuinen
    • 2
  • Anna Dvořáčková
    • 1
  • Marie Tollot
    • 2
  • Vivienne Gianinazzi-Pearson
    • 2
  • Miroslav Vosátka
    • 1
  • Armelle Gollotte
    • 2
  1. 1.Institute of Botany, Academy of Sciences of the Czech RepublicPrůhoniceCzech Republic
  2. 2.UMR 1088 INRA/5184 CNRS/Université de Bourgogne, Plante-Microbe-EnvironnementDijon CedexFrance