Mycorrhiza

, Volume 17, Issue 4, pp 327–335

GintMT1 encodes a functional metallothionein in Glomus intraradices that responds to oxidative stress

  • M. González-Guerrero
  • C. Cano
  • C. Azcón-Aguilar
  • N. Ferrol
Original Paper

DOI: 10.1007/s00572-007-0108-7

Cite this article as:
González-Guerrero, M., Cano, C., Azcón-Aguilar, C. et al. Mycorrhiza (2007) 17: 327. doi:10.1007/s00572-007-0108-7

Abstract

A full-length metallothionein (MT) gene (GintMT1) was isolated from Glomus intraradices extraradical mycelium. This is the first MT gene reported in the genus Glomus, third in the Glomeromycota. Functional analysis of GintMT1 in a MT-defective Saccharomyces cerevisiae strain indicates that it encodes a functional MT. Gene expression analyses revealed that the transcript levels of GintMT1 were elevated in mycelia treated with 5 mM Cu or paraquat but inhibited in mycelia treated with 50 μM Cu or 450 μM Cd. The elevated expression of GintMT1 in the 5 mM Cu-treated mycelia together with the ability of GintMT1 to provide tolerance to a Cu-sensitive yeast suggests that GintMT1 might afford protection against Cu. Induction of GintMT1 expression by paraquat and 5 mM Cu, treatments that also produced an oxidative damage to the fungal membranes, suggests that GintMT1 may play a role in the regulation of the redox status of the extraradical mycelium of G. intraradices.

Keywords

Arbuscular mycorrhizal fungiHeavy metalsLipid peroxidationMetallothioneinOxidative stress

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • M. González-Guerrero
    • 1
  • C. Cano
    • 1
  • C. Azcón-Aguilar
    • 1
  • N. Ferrol
    • 1
  1. 1.Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del ZaidínConsejo Superior de Investigaciones CientíficasGranadaSpain