Microsystem Technologies

, Volume 12, Issue 1, pp 120–127

Bio-MEMS fabricated artificial capillaries for tissue engineering

  • G. J. Wang
  • C. L. Chen
  • S. H. Hsu
  • Y. L. Chiang
Technical paper

DOI: 10.1007/s00542-005-0017-7

Cite this article as:
Wang, G.J., Chen, C.L., Hsu, S.H. et al. Microsyst Technol (2005) 12: 120. doi:10.1007/s00542-005-0017-7

Abstract

In this report, we focus on the microfabrication and cell seeding issues of artificial blood capillaries for tissue engineering. Two different fabrication methods (stainless steel electroforming and silicon electroforming) and a number of materials (PC, Polycarbonate and biocompatible material PLGA, poly lactide-co-glycolides) are implemented to build the vascular network. The vascular network is then used as the scaffold to cultivate the bovine endothelial cell (BEC). During the period of cell cultivation, oxygen and nutrient need to be continuously delivered by a circular pressurizing system. In cell culture, encouraging results are obtained through the dynamical seeding of the BEC on the scaffolds. A systematic cell culture process has been developed after repeated experiments. Successful seeding efficiencies are obtained by using the developed systematic cell culture process.

Copyright information

© Springer-Verlag 2005

Authors and Affiliations

  • G. J. Wang
    • 1
  • C. L. Chen
    • 1
  • S. H. Hsu
    • 2
  • Y. L. Chiang
    • 2
  1. 1.Department of Mechanical EngineeringNational Chung-Hsing UniversityTaichungTaiwan
  2. 2.Department of Chemical EngineeringNational Chung-Hsing UniversityTaichungTaiwan