Journal of Gastroenterology

, Volume 36, Issue 8, pp 544–551

Th1/Th2 cytokine profiles and their relationship to clinical features in patients with chronic hepatitis C virus infection

Authors

  • Satoshi Sobue
    • First Department of Internal Medicine, Medical School, Nagoya City University, Nagoya, Japan
  • Tomoyuki Nomura
    • First Department of Internal Medicine, Medical School, Nagoya City University, Nagoya, Japan
  • Tetsuya Ishikawa
    • First Department of Internal Medicine and Research Center for Infections Disease, Aichi Medical University, 21 Karimata Yazako, Nagakute-cho, Aichi 480-1195, Japan
  • Satomi Ito
    • First Department of Internal Medicine and Research Center for Infections Disease, Aichi Medical University, 21 Karimata Yazako, Nagakute-cho, Aichi 480-1195, Japan
  • Katsuhisa Saso
    • First Department of Internal Medicine, Medical School, Nagoya City University, Nagoya, Japan
  • Hirotaka Ohara
    • First Department of Internal Medicine, Medical School, Nagoya City University, Nagoya, Japan
  • Takashi Joh
    • First Department of Internal Medicine, Medical School, Nagoya City University, Nagoya, Japan
  • Makoto Itoh
    • First Department of Internal Medicine, Medical School, Nagoya City University, Nagoya, Japan
  • Shinichi Kakumu
    • First Department of Internal Medicine and Research Center for Infections Disease, Aichi Medical University, 21 Karimata Yazako, Nagakute-cho, Aichi 480-1195, Japan

DOI: 10.1007/s005350170057

Cite this article as:
Sobue, S., Nomura, T., Ishikawa, T. et al. J Gastroenterol (2001) 36: 544. doi:10.1007/s005350170057

Purpose.

An imbalance in helper T-cell type 1 (Th1) and type 2 (Th2) cytokines is suggested to play an important role in the pathogenesis of chronic viral infections, but this issue is not resolved in patients with hepatitis C virus (HCV) infection. The aim of this study was to clarify the relationship between the balance of Th1 and Th2 cytokines and liver damage. Methods. We investigated cytokine levels in the peripheral blood and liver tissue of patients with chronic HCV infection (n = 59) by three different methods; we used flow cytometry to detect intracellular cytokines, and we measured cytokine titers in sera and in the supernatants of lymphocyte cultures with enzyme-linked immunosorbent assays (ELISAs). Results. In both CD4+ and CD8+ cells, interferon (IFN) γ-producing cell populations increased, while there was no difference in interleukin (IL)-10 production, indicating a shift to a Th1 cytokine profile with the progression of liver disease. With respect to the ratio of IFN-γ to IL-10, a correlation was found in CD4+ cells between peripheral blood and liver tissue (r = 0.98; P = 0.0011). Th1 cytokine was predominant in intrahepatic CD4+ cells, while it was predominant in peripheral blood CD8+ cells. Conclusions. These findings indicate a correlation between dominant Th1 response and disease activity and progression. In addition, we suggest that intrahepatic CD4+ T cells play a pathogenetic role in the hepatic injury of HCV infection.

Key words: immunopathogenesisinterferon gammainterleukin-10hepatitis C virus
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Copyright information

© Springer-Verlag Tokyo 2001