Journal of Gastroenterology

, Volume 35, Issue 9, pp 682–689

Anti-cathepsin G antibodies in the sera of patients with ulcerative colitis

Authors

  • Toshimitsu Kuwana
    • Second Department of Internal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan
  • Yukio Sato
    • Second Department of Internal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan
  • Mitsuru Saka
    • Second Department of Internal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan
  • Yuichiro Kondo
    • Second Department of Internal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan
  • Masayuki Miyata
    • Second Department of Internal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan
  • Katsutoshi Obara
    • Second Department of Internal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan
  • Tomoe Nishimaki
    • Second Department of Internal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan
  • Reiji Kasukawa
    • Second Department of Internal Medicine, Fukushima Medical University School of Medicine, 1 Hikarigaoka, Fukushima 960-1295, Japan

DOI: 10.1007/s005350070047

Cite this article as:
Kuwana, T., Sato, Y., Saka, M. et al. J Gastroenterol (2000) 35: 682. doi:10.1007/s005350070047

Abstract:

The presence of perinuclear anti-neutrophil cytoplasmic antibodies (P-ANCAs) and that of antibodies against cathepsin G, a target antigen for P-ANCAs, was determined in the sera of patients with ulcerative colitis (UC), relative to the endoscopic severity and disease activity. P-ANCAs were detected by indirect immunofluorescent assay (IIF) on ethanol-fixed human neutrophils. Antibodies to cathepsin G were detected by an enzyme-linked immunosorbent assay (ELISA) and Western blotting. P-ANCAs were detected by IIF in 62.5% of 32 patients with active UC. Anti-cathepsin G antibodies were detected in 40.6% of 32 patients with active UC, and their prevalence was significantly higher in patients with severe colitis, as determined by endoscopy, than in those with mild or moderate colitis (P < 0.05). The prevalence and titers of anti-cathepsin G antibodies were significantly higher during the active than the inactive phase of the disease (P < 0.05). Measurement of titers of anti-cathepsin G antibodies by ELISA in the serum is useful for evaluating the activity of UC.

Key words: anti-cathepsin G antibodies antineutrophil antibodies ulcerative colitis serology

Copyright information

© Springer-Verlag Tokyo 2000