IL28B polymorphism is associated with fatty change in the liver of chronic hepatitis C patients
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- Ohnishi, M., Tsuge, M., Kohno, T. et al. J Gastroenterol (2012) 47: 834. doi:10.1007/s00535-012-0550-y
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Several single nucleotide polymorphisms (SNPs) within the interleukin 28B (IL28B) locus are associated with sustained viral response in chronic hepatitis C (HCV) patients who were treated with pegylated interferon (PEG-IFN) plus ribavirin (RBV) combination therapy. Recently, an association between γ-GTP level and IL28B genotype was identified. In this study, the relationship between IL28B genotype and liver steatosis was analyzed.
One hundred fifty-three patients who underwent liver biopsy before PEG-IFN plus RBV combination therapy were enrolled. The level of liver steatosis was measured using a BIOREVO BZ-9000 microscope, and the proportion of fatty change and clear cell change were calculated using Dynamic cell count BZ-H1C software. IL28B SNP genotype (rs8099917) was determined using the Invader Assay.
Vesicular change was significantly associated with body mass index (BMI), HCV RNA titer, serum aspartate aminotransferase, γ-GTP, IL28B genotype and liver fibrosis level (P < 0.05). Clear cell change was significantly associated with serum aspartate aminotransferase, γ-GTP and IL28B genotype by univariate logistic regression analysis (P < 0.05). Under multiple logistic regression, IL28B genotype (ORadj = 8.158; 95% CI 2.412–27.589), liver fibrosis (ORadj = 2.541; 95% CI 1.040–6.207) and BMI (ORadj = 1.147; 95% CI 1.011–1.301) were significant independent factors for vesicular change and IL28B genotype (ORadj = 3.000; 95% CI 1.282–7.019) for clear cell change.
In this study, a new quantitative method to objectively evaluate hepatic steatosis was described. IL28B genotypes were significantly associated with both vesicular and clear cell changes of livers in chronic hepatitis C patients.
KeywordsHCV Core substitution IL28B Fatty change SNP
Hepatitis C virus
IFN-sensitivity determining region
Single nucleotide polymorphism
Body mass index
Gamma glutamyl transpeptidase
Homeostasis model assessment of insulin resistance