Journal of Gastroenterology

, Volume 46, Issue 12, pp 1391–1402

Differential expression of miR-144* as a novel fecal-based diagnostic marker for colorectal cancer

  • Murugan Kalimutho
  • Giovanna Del Vecchio Blanco
  • Serena Di Cecilia
  • Pierpaolo Sileri
  • Micaela Cretella
  • Francesco Pallone
  • Giorgio Federici
  • Sergio Bernardini
Original Article—Alimentary Tract

DOI: 10.1007/s00535-011-0456-0

Cite this article as:
Kalimutho, M., Del Vecchio Blanco, G., Di Cecilia, S. et al. J Gastroenterol (2011) 46: 1391. doi:10.1007/s00535-011-0456-0

Abstract

Background

MicroRNAs (miRNA) are tiny, noncoding, small, endogenous RNAs that play major roles in neoplastic transformation and could therefore offer a better quantitative and noninvasive method for the diagnosis and prognosis of colorectal cancer (CRC) using feces. In the present study, we screened feces for 648 miRNAs and analyzed the role of miR-144* as a potential CRC diagnostic marker.

Methods

Fecal miRNA expression was profiled with RT-pre-amplification-qPCR, and the stability was determined using both endogenous and exogenous miRNA by RT-qPCR. ROC analysis was performed to enhance the diagnosing power of the CRC patients’ fecal specimens.

Results

We detected 39% of all the miRNAs screened in feces. Endogenous miRNAs are more stable over time and temperature, while exogenous miRNAs degraded rapidly. miR-144* was overexpressed in feces, suggesting that it could be a potent candidate diagnostic marker for CRC detection, with a sensitivity of 74% and a specificity of 87% (n = 75, p < 0.0001). Moreover, RT-qPCR analysis showed that miR-144* was also overexpressed in paired CRC tissues, thus suggesting its possible utilization as a diagnostic marker.

Conclusions

We demonstrated that miRNAs are stable in the fecal microenvironment, and that, among them, miR-144* represents a novel fecal-based diagnostic marker for CRC screening. Nevertheless, our data need to be validated in a large cohort of subjects.

Keywords

miR-144*Fecal miRNAsCRCmiR-378

Abbreviations

miRNA

MicroRNA

fDNA

Fecal DNA

CRC

Colorectal cancer

Copyright information

© Springer 2011

Authors and Affiliations

  • Murugan Kalimutho
    • 1
    • 4
  • Giovanna Del Vecchio Blanco
    • 2
  • Serena Di Cecilia
    • 1
  • Pierpaolo Sileri
    • 2
    • 3
  • Micaela Cretella
    • 2
  • Francesco Pallone
    • 1
    • 2
  • Giorgio Federici
    • 1
  • Sergio Bernardini
    • 1
  1. 1.Department of Internal MedicineUniversity of Rome “Tor Vergata”RomeItaly
  2. 2.Gastroenterology UnitUniversity Hospital Tor Vergata, University of Rome Tor VergataRomeItaly
  3. 3.Surgical UnitUniversity Hospital Tor Vergata, University of Rome Tor VergataRomeItaly
  4. 4.Centre for Cancer Research and Cell Biology (CCRCB)Queen’s UniversityBelfastNorthern Ireland, UK