Mechanism of interdigestive migrating motor complex in conscious dogs
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The migrating motor complex (MMC) is well characterized by the appearance of gastrointestinal contractions in the interdigestive state. This study was designed to clarify the mechanisms of gastric MMC (G-MMC) and intestinal MMC (I-MMC) in conscious dogs.
Five strain gauge transducers were implanted on the stomach and intestine. To investigate the correlation between luminal 5-HT and phase III contractions, gastric and duodenal juices were collected during the MMC cycle. The 5-HT concentrations in gastric and duodenal juice were measured by HPLC. To investigate whether luminal 5-HT initiates MMC, 5-HT (10−8–10−6 M, 10 ml) was administered into the duodenum 20 min after gastric phase III. To investigate the involvement of 5-HT3 or 5-HT4 receptors in mediating G-MMC and I-MMC, 5-HT3 antagonists (ondansetron) or 5-HT4 antagonists (GR 125,487) were infused for 120 min.
Luminal administration of 5-HT (10−6 M) initiated duodenal phase II followed by G-MMC and I-MMC with a concomitant increased release of plasma motilin. The duodenal 5-HT concentration was significantly increased during phase II (59 ± 9 ng/ml) and phase III (251 ± 21 ng/ml) compared to that of phase I (29 ± 5 ng/ml). On the other hand, the 5-HT content in the stomach was not significantly changed throughout the MMC cycle. Intravenous infusion of motilin (0.3 μg/kg/h) increased the luminal 5-HT content and induced G-MMC and I-MMC. 5-HT4 antagonists significantly inhibited both G-MMC and I-MMC, while 5-HT3 antagonists inhibited only G-MMC.
It is suggested that the MMC cycle is mediated by a positive feedback mechanism via the interaction between motilin and 5-HT.
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- Mechanism of interdigestive migrating motor complex in conscious dogs
Journal of Gastroenterology
Volume 45, Issue 5 , pp 506-514
- Cover Date
- Print ISSN
- Online ISSN
- Springer Japan
- Additional Links
- Gastric MMC
- Intestinal MMC
- Industry Sectors
- Author Affiliations
- 1. Department of Surgery, Duke University Medical Center, Durham, NC, USA
- 2. Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, USA
- 3. Research Service 151, Zablocki VA Medical Center, 5000 West National Avenue, Milwaukee, WI, 53295, USA